Exploring the Molecular Mechanism for Color Distinction in Humans
| Autor: | Nagarajan Vaidehi, William A. Goddard, Rene J. Trabanino |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Models
Molecular Rhodopsin Opsin Absorption spectroscopy genetic structures Protein Conformation Stereochemistry Molecular Sequence Data Molecular dynamics Protein structure Materials Chemistry Animals Humans Amino Acid Sequence Homology modeling Physical and Theoretical Chemistry Schiff Bases biology Chemistry Spectrophotometry Atomic Rod Opsins Chromophore Surfaces Coatings and Films Retinaldehyde Biophysics biology.protein Quantum Theory Cattle sense organs Sequence Alignment Color Perception Protein Binding |
| Popis: | We examine here the role of the red, green, and blue human opsin structures in modulating the absorption properties of 11-cis-retinal bonded to the protein via a protonated Schiff base (PSB). We built the three-dimensional structures of the human red, green, and blue opsins using homology modeling techniques with the crystal structure of bovine rhodopsin as the template. We then used quantum mechanics (QM) combined with molecular mechanics (MM) (denoted as QM/MM) techniques in conjunction with molecular dynamics to determine how the room temperature molecular structures of the three human color opsin proteins modulate the absorption frequency of the same bound 11-cis-retinal chromophore to account for the differences in the observed absorption spectra. We find that the conformational twisting of the 11-cis-retinal PSB plays an important role in the green to blue opsin shift, whereas the dipolar side chains in the binding pocket play a surprising role of red-shifting the blue opsin with respect to the green opsin, as a fine adjustment to the opsin shift. The dipolar side chains play a large role in the opsin shift from red to green. |
| Databáze: | OpenAIRE |
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