Role of membrane-anchored heparin-binding epidermal growth factor-like growth factor and CD9 on macrophages
| Autor: | Shizuya Yamashita, Tohru Funahashi, Sumio Kawata, Yuji Matsuzawa, Shigeki Higashiyama, Tsutomu Nakagawa, Noriyuki Ouchi, Iichiro Shimomura, Naoyuki Taniguchi, Kaoru Kameda-Takemura, Shinji Kihara |
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| Rok vydání: | 1997 |
| Předmět: |
Heparin-binding EGF-like growth factor
Cellular differentiation medicine.medical_treatment Biochemistry Muscle Smooth Vascular Tetraspanin 29 Cell Line chemistry.chemical_compound Antigens CD Epidermal growth factor medicine Humans RNA Messenger Protein Precursors Molecular Biology Membrane Glycoproteins Epidermal Growth Factor biology Macrophages Growth factor Lysophosphatidylcholines Cell Differentiation DNA Cell Biology Flow Cytometry Juxtacrine signalling Cell biology Lipoproteins LDL Membrane glycoproteins Lysophosphatidylcholine chemistry Cell culture embryonic structures biology.protein Intercellular Signaling Peptides and Proteins hormones hormone substitutes and hormone antagonists Heparin-binding EGF-like Growth Factor Research Article |
| Zdroj: | Biochemical Journal. 328:923-928 |
| ISSN: | 1470-8728 0264-6021 |
| Popis: | Heparin-binding epidermal-growth-factor-like growth factor (HB-EGF) is a potent mitogen for smooth-muscle cells (SMCs) belonging to the EGF family. We have previously determined that HB-EGF is expressed in macrophages and SMCs of human atherosclerotic lesions and that its membrane-anchored precursor, proHB-EGF, also has a juxtacrine mitogenic activity which is markedly enhanced by CD9, a surface marker of lymphohaemopoietic cells. Therefore, when both proHB-EGF and CD9 are expressed on macrophages, they may strongly promote the development of atherosclerosis. In the present study we have investigated the changes in proHB-EGF and CD9 in THP-1 cells during differentiation into macrophages and by the addition of oxidized low-density lipoproteins (OxLDL) and assessed juxtacrine growth activity of THP-1 macrophages for human aortic SMCs. HB-EGF and CD9 at both the mRNA and the protein level were up-regulated after differentiation into macrophages, and further expression of HB-EGF was induced by the addition of OxLDL or lysophosphatidylcholine. Juxtacrine induction by formalin-fixed growth was suppressed to control levels by an inhibitor of HB-EGF and was partially decreased by anti-CD9 antibodies. These results suggest that co-expression of proHB-EGF and CD9 on macrophages plays an important role in the development of atherosclerosis by a juxtacrine mechanism. |
| Databáze: | OpenAIRE |
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