Filopodia-like protrusions of adjacent somatic cells shape the developmental potential of oocytes

Autor: Flora Crozet, Gaëlle Letort, Rose Bulteau, Christelle Da Silva, Adrien Eichmuller, Anna Francesca Tortorelli, Joséphine Blévinal, Morgane Belle, Julien Dumont, Tristan Piolot, Aurélien Dauphin, Fanny Coulpier, Alain Chédotal, Jean-Léon Maître, Marie-Hélène Verlhac, Hugh J Clarke, Marie-Emilie Terret
Přispěvatelé: Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de recherche de l'Institut Curie [Paris], Institut Curie [Paris], Institut Jacques Monod (IJM (UMR_7592)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut de la Vision, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Génétique et Biologie du Développement, Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Biologie du système neuromusculaire - Biology of the neuromuscular system [Maisons-Alfort] (BNMS - Team 10), École nationale vétérinaire - Alfort (ENVA)-Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), The STED microscopy was performed at the Orion Platform (member of France-Bioimaging ANR-10-INBS-XX) of the Center for Interdisciplinary Research in Biology (UMR7241/U1050) of Collège de France. The OMX microscopy was performed at the Cell and Tissue Imaging Platform-PICT-IBiSA of the Genetics and Developmental Biology Department (UMR3215/U934) of Institut Curie. This work was supported by the Fondation pour la Recherche Médicale (FRM Label EQU201903007796 to M-H Verlhac), by the Agence Nationale de la Recherche (ANR-18-CE13 to M-H Verlhac and Auguste Genovesio, IBENS/ENS, ANR-16-CE13 to M-E Terret), and by the France Canada Research Fund (FCRF 2017 to M-E Terret and Hugh J Clarke, McGill University). F Crozet obtained a grant from the Fondation pour la Recherche Médicale for her fourth PhD year (FDT202001010906). This work has received support from the Fondation Bettencourt Schueller.., ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), ANR-10-INBS-0009,France-Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), ANR-10-LABX-0054,MEMOLIFE,Memory in living systems: an integrated approach(2010), ANR-10-IDEX-0001,PSL,Paris Sciences et Lettres(2010), European Project: 250367,EC:FP7:ERC,ERC-2009-AdG,EPIGENETIX(2010)
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Life Science Alliance
Life Science Alliance, 2023, 6 (6), pp.e202301963. ⟨10.26508/lsa.202301963⟩
ISSN: 2575-1077
DOI: 10.26508/lsa.202301963⟩
Popis: The oocyte must grow and mature before fertilization, thanks to a close dialogue with the somatic cells that surround it. Part of this communication is through filopodia-like protrusions, called transzonal projections (TZPs), sent by the somatic cells to the oocyte membrane. To investigate the contribution of TZPs to oocyte quality, we impaired their structure by generating a full knockout mouse of the TZP structural component myosin-X (MYO10). Using spinning disk and super-resolution microscopy combined with a machine-learning approach to phenotype oocyte morphology, we show that the lack ofMyo10decreases TZP density during oocyte growth. Reduction in TZPs does not prevent oocyte growth but impairs oocyte-matrix integrity. Importantly, we reveal by transcriptomic analysis that gene expression is altered in TZP-deprived oocytes and that oocyte maturation and subsequent early embryonic development are partially affected, effectively reducing mouse fertility. We propose that TZPs play a role in the structural integrity of the germline–somatic complex, which is essential for regulating gene expression in the oocyte and thus its developmental potential.
Databáze: OpenAIRE
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