Localization of Alzheimer-Associated Presenilin 1 in Transfected COS-7 Cells
| Autor: | Miyuki Murayama, Michio Sato, Yuko Nakazato, Toshiyuki Honda, Hiroshi Takahashi, Marc Mercken, Shouji Tanaka, Akihiko Takashima, Kazuki Sato, Kaori Noguchi, Shunzo Kondo |
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| Rok vydání: | 1996 |
| Předmět: |
Cell signaling
Biophysics Cell Communication Biology Endoplasmic Reticulum Transfection Biochemistry Presenilin Cell membrane Alzheimer Disease Chlorocebus aethiops mental disorders Presenilin-1 medicine Animals Humans Microscopy Immunoelectron Molecular Biology Cell Nucleus Chromosomes Human Pair 14 COS cells Cell adhesion molecule Endoplasmic reticulum Cell Membrane Antibodies Monoclonal Membrane Proteins STIM1 Cell Biology Molecular biology Recombinant Proteins nervous system diseases Cell biology medicine.anatomical_structure COS Cells Cell Adhesion Molecules |
| Zdroj: | Biochemical and Biophysical Research Communications. 227:423-426 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1996.1523 |
| Popis: | Presenilin 1 (PS 1) is the recently identified gene, located on chromosome 14, of which missense mutations can cause early-onset familial Alzheimer's disease. To understand the normal biological function of presenilin 1, we examined the sub-cellular localization by using a monoclonal anti-presenilin 1 antibody. Immuno-electronmicroscopic and biochemical analysis indicated that presenilin 1 is localized on cellular membrane (plasma, endoplasmic reticulum, and perinuclear) in COS-7 cells overexpressing presenilin 1. Interestingly, the PS 1 immunoreactivity in the plasma membrane was concentrated in the regions with cell-cell contact. This observation suggests a possible role of PS 1 on the cell membrane as a cell adhesion molecule. |
| Databáze: | OpenAIRE |
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