Li+ effect on the cell wall of the yeast Saccharomyces cerevisiae as probed by FT-IR spectroscopy
| Autor: | Audrius Misiūnas, Larisa Chaustova, Aurelijus Zimkus |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
General Immunology and Microbiology Strain (chemistry) biology Chemistry QH301-705.5 General Neuroscience transformation Saccharomyces cerevisiae Analytical chemistry biology.organism_classification General Biochemistry Genetics and Molecular Biology Yeast Ion Cell wall Crystallography ft-ir spectroscopy Absorption band lithium saccharomyces cerevisiae Biology (General) General Agricultural and Biological Sciences Spectroscopy Glucan |
| Zdroj: | Open Life Sciences, Vol 8, Iss 8, Pp 724-729 (2013) |
| ISSN: | 2391-5412 |
| Popis: | The effect of Li+ ions as a transformation inducing agent on the yeast cell wall has been studied. Two Saccharomyces cerevisiae strains, p63-DC5 with a native cell wall, and strain XCY42-30D(mnn1) which contains structural changes in the mannan-protein complex, were used. Fourier transform infrared (FT-IR) spectroscopy has been used for the characterization of the yeast strains and for determination of the effect of lithium cations on the cell wall. A comparison of the carbohydrate absorption band positions in the 970–1185 cm−1 range, of Na+ and Li+ treated yeast cells has been estimated. Absorption band positions of the cell wall carbohydrates of p63-DC5 were not influenced by the studied ions. On the contrary, the treatment of XCY42-30D(mnn1) cells with Li+ ions shifted glucan band positions, implying that the cell wall structure of strain XCY42-30D(mnn1) is more sensitive to Li+ ion treatment. |
| Databáze: | OpenAIRE |
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