Dendritic cells are dysfunctional in patients with operable breast cancer
| Autor: | David M. Clark, Oleg Eremin, Jibril A Jibril, Wichai Vassanasiri, Sukchai Satthaporn, David Valerio, Adrian Robins, Mohamed El-Sheemy |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Cancer Research T-Lymphocytes T cell Immunology Breast Neoplasms chemical and pharmacologic phenomena Cell Separation Lymphocyte Activation Tuberculin Breast cancer Antigen Antigens CD medicine Humans Immunology and Allergy Cytotoxic T cell Antigen-presenting cell Lymph node Aged Aged 80 and over CD86 Antigen Presentation Membrane Glycoproteins business.industry hemic and immune systems Dendritic Cells HLA-DR Antigens Dendritic cell Middle Aged medicine.disease Interleukin-12 medicine.anatomical_structure Oncology Case-Control Studies Female B7-2 Antigen Lymph Nodes Lymphocyte Culture Test Mixed business Cell Division |
| Zdroj: | Cancer Immunology, Immunotherapy. 53:510-518 |
| ISSN: | 1432-0851 0340-7004 |
| DOI: | 10.1007/s00262-003-0485-5 |
| Popis: | Background: Dendritic cells (DCs) play a crucial role in presenting antigens to T lymphocytes and inducing cytotoxic T cells. DCs have been studied in patients with breast cancer to define the factors leading to failure of an effective systemic and locoregional anticancer host response. Methods: Purified DCs were obtained from peripheral blood (PB) and lymph nodes (LNs) of women with operable breast cancer, using immunomagnetic bead selection. The stimulatory capacity of DCs in the allogeneic mixed leukocyte reaction (MLR) and autologous T cell proliferation test (purified protein derivative (PPD) as stimulator), the expression of surface markers on DCs and the production of cytokines in vitro by DCs from patients with operable breast cancer and from healthy donors (controls) were studied. Results: 70–75% purified DCs were isolated from PB and LNs. PBDCs and LNDCs from patients with operable breast cancer demonstrated a reduced capacity to stimulate in an MLR, compared with PBDCs from normal donors (p |
| Databáze: | OpenAIRE |
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