A Molecular Network for the Transport of the TI-VAMP/VAMP7 Vesicles from Cell Center to Periphery
| Autor: | Andrea Burgo, Etienne Formstecher, Philippe Bun, Thierry Galli, Maïté Coppey-Moisan, Véronique Proux-Gillardeaux, Ronald K.H. Liem, Agathe Verraes, Emmanuel Sotirakis, Alessandra Maria Casano |
|---|---|
| Přispěvatelé: | Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), INSERM ERL U950, Membrane Traffic in Neuronal and Epithelial Morphogenesis, Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Pathology and Cell Biology, Taub Institute for Research on Alzheimer's Disease and Aging Brain, Hybrigenics [Paris], Hybrigenics, Association Francaise contre les Myopathies (AFM), Association pour la Recherche sur le Cancer (ARC), Mairie de Paris Medical Research and Health Program, Fondation pour la Recherche Medicale (FRM), Ecole des Neurosciences de Paris (ENP) |
| Jazyk: | angličtina |
| Předmět: |
MESH: Protein Transport
MESH: R-SNARE Proteins Growth Cones Golgi Apparatus Kinesins [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology General Biochemistry Genetics and Molecular Biology Exocytosis MESH: Nocodazole R-SNARE Proteins 03 medical and health sciences MESH: Golgi Apparatus 0302 clinical medicine Microtubule Chlorocebus aethiops MESH: RNA Small Interfering MESH: Tubulin Modulators Animals Humans MESH: Animals RNA Small Interfering Molecular Biology [SDV.BDD]Life Sciences [q-bio]/Development Biology Actin 030304 developmental biology 0303 health sciences MESH: Humans Nocodazole Vesicle Cell Biology MESH: Growth Cones Secretory Vesicle MESH: Cercopithecus aethiops Tubulin Modulators Cell biology MESH: COS Cells Protein Transport MACF1 COS Cells MESH: HeLa Cells Kinesin Guanine nucleotide exchange factor MESH: Kinesin 030217 neurology & neurosurgery HeLa Cells Developmental Biology |
| Zdroj: | Developmental Cell Developmental Cell, Elsevier, 2012, 23 (1), pp.166-80. ⟨10.1016/j.devcel.2012.04.019⟩ ResearcherID |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2012.04.019 |
| Popis: | International audience; The compartmental organization of eukaryotic cells is maintained dynamically by vesicular trafficking. SNARE proteins play a crucial role in intracellular membrane fusion and need to be targeted to their proper donor or acceptor membrane. The molecular mechanisms that allow for the secretory vesicles carrying the v-SNARE TI-VAMP/VAMP7 to leave the cell center, load onto microtubules, and reach the periphery to mediate exocytosis are largely unknown. Here, we show that the TI-VAMP/VAMP7 partner Varp, a Rab21 guanine nucleotide exchange factor, interacts with GolginA4 and the kinesin 1 Kif5A. Activated Rab21-GTP in turn binds to MACF1, an actin and microtubule regulator, which is itself a partner of GolginA4. These components are required for directed movement of TI-VAMP/VAMP7 vesicles from the cell center to the cell periphery. The molecular mechanisms uncovered here suggest an integrated view of the transport of vesicles carrying a specific v-SNARE toward the cell surface. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|