Hypoxia-inducible factor-1α has a key role in hypoxic preconditioning
| Autor: | Shuhei Tomita, Kosuke Chujo, Junichiro Ono, Takehiko Asaga, Masaaki Ueki, Yasuyuki Iwanaga, Satoshi Taie |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Partial Pressure Mutant Gene Expression Nitric Oxide Synthase Type II chemistry.chemical_element Polymerase Chain Reaction Oxygen Mice Downregulation and upregulation Transcription (biology) Physiology (medical) Gene expression medicine Animals Hypoxia Ischemic Preconditioning Mice Knockout Analysis of Variance biology business.industry Brain General Medicine Hypoxia (medical) Hypoxia-Inducible Factor 1 alpha Subunit Cell biology Nitric oxide synthase Neurology Biochemistry Hypoxia-inducible factors chemistry biology.protein Surgery Neurology (clinical) medicine.symptom business |
| Zdroj: | Journal of Clinical Neuroscience. 16:1056-1060 |
| ISSN: | 0967-5868 |
| DOI: | 10.1016/j.jocn.2008.09.024 |
| Popis: | Sublethal hypoxia induces tolerance to subsequent hypoxic insults in a process known as hypoxic preconditioning (HP). Hypoxia-inducible factor-1 alpha (HIF-1 alpha) is a key transcription protein involved in the mechanism of HP. In this study, we investigated the effects of HP on tissue oxygenation and expression of HIF-1 alpha gene targets in the brain using neural cell-specific HIF-1 alpha-deficient mice. The animals were exposed to 8% oxygen for 3 hours. Twenty-four hours later, the oxygen partial pressure (pO(2)) of brain tissue and gene expression were measured during hypoxia. HP improved the pO(2) of brain tissue during subsequent hypoxia with upregulated inducible nitric oxide synthase in wild-type mice, whereas HP had no detectable effect in the mutant mice. Our results indicate that the protective effects of HP may be partially mediated by improving tissue oxygenation via HIF-1 alpha and inducible nitric oxide synthase. |
| Databáze: | OpenAIRE |
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