Identification of crucial noncoding RNAs and mRNAs in hypertrophic scars via RNA sequencing
| Autor: | Yan Han, Zeliang He, Julei Zhang, Xiaodong Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
ceRNA network Cicatrix Hypertrophic QH301-705.5 Biology General Biochemistry Genetics and Molecular Biology Collagen fibril organization Extracellular matrix 03 medical and health sciences Hypertrophic scar 0302 clinical medicine lncRNA microRNA medicine Humans circRNA RNA Messenger Biology (General) Research Articles MEG3 Competing endogenous RNA Sequence Analysis RNA RNA hypertrophic scar RNA Circular medicine.disease Cell biology 030104 developmental biology 030220 oncology & carcinogenesis RNA Long Noncoding Signal transduction Research Article |
| Zdroj: | FEBS Open Bio FEBS Open Bio, Vol 11, Iss 6, Pp 1673-1684 (2021) |
| ISSN: | 2211-5463 |
| Popis: | Hypertrophic scarring (HS) is a dermal fibroproliferative disorder characterized by excessive deposition of collagen and other extracellular matrix components. The aim of this study is to explore crucial long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) associated with HS and provide a better understanding of the molecular mechanism of HS. To investigate the lncRNA, circRNA and mRNA expression profiles, we performed RNA sequencing of human HS and normal skin tissues. After the identification of differentially expressed mRNAs (DEmRNAs), lncRNAs (DElncRNAs) and circRNAs (DEcircRNAs), we performed functional enrichment of DEmRNAs. Further on, we constructed DElncRNA/DEcircRNA–DEmRNA coexpression networks and competing endogenous RNA regulatory networks, and performed functional analyses of the DEmRNAs in the constructed networks. In total, 487 DEmRNAs, 92 DElncRNAs and 17 DEcircRNAs were identified. DEmRNAs were significantly enriched in processes such as collagen fibril organization, extracellular matrix–receptor interaction and the phosphatidylinositol 3‐kinase (PI3K)–Akt signaling pathway. In addition, we detected 580 DElncRNA–DEmRNA and 505 DEcircRNA–DEmRNA coexpression pairs. The competing endogenous RNA network contained 18 circRNA–microRNA (miRNA) pairs, 18 lncRNA–miRNA pairs and 409 miRNA–mRNA pairs, including 10 circRNAs, 5 lncRNAs, 15 miRNAs and 160 mRNAs. We concluded that MIR503HG/hsa‐miR‐204‐3p/ACAN, MIR503HG/hsa‐miR‐431‐5p/TNFRSF9, MEG3/hsa‐miR‐6884‐5p/ADAMTS14, AC000035.1‐ADAMTS14 and hsa_circ_0069865‐COMP/ADAM12 interaction pairs may play a central role in HS. We performed RNA sequencing to acquire the expression profiles of long noncoding RNA, circular RNA and mRNA in human hypertrophic scar (HS) and normal skin tissues from three male patients with HS. We concluded that MIR503HG/hsa‐miR‐204‐3p/ACAN, MIR503HG/hsa‐miR‐431‐5p/TNFRSF9, MEG3/hsa‐miR‐6884‐5p/ADAMTS14, AC000035.1‐ADAMTS14 and hsa_circ_0069865‐COMP/ADAM12 interaction pairs may play a central role in HS. |
| Databáze: | OpenAIRE |
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