Comparative study between two techniques for artificial shrinkage of blastocysts prior to vitrification: LASER pulse versus micro-needle technique in increasing chemical, clinical pregnancy and live birth rates after ICSI, a randomized controlled trial
| Autor: | Mohamed Amr. H. Elnoury, Medhat Amer, Manal Kamel Mohamed, Mahmoud Alalfy, Emad Fakhry |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Pregnancy Rate Reproductive medicine Cryopreservation law.invention 03 medical and health sciences 0302 clinical medicine Randomized controlled trial Pregnancy law medicine Humans Vitrification Sperm Injections Intracytoplasmic Fertility preservation Birth Rate reproductive and urinary physiology Shrinkage 030219 obstetrics & reproductive medicine Pulse (signal processing) business.industry Obstetrics Lasers Obstetrics and Gynecology Abortion Spontaneous Blastocyst 030104 developmental biology Pediatrics Perinatology and Child Health Female business Live birth |
| Zdroj: | The Journal of Maternal-Fetal & Neonatal Medicine. 35:4910-4917 |
| ISSN: | 1476-4954 1476-7058 |
| Popis: | This work investigates ICSI outcome between LASER Artificial Shrinkage (LAS) and Micro-Needle Artificial Shrinkage (MNAS) before vitrification.Four hundred and nine women were included in the study; which were randomly divided into two groups according to the technique used for artificial shrinkage step of the blastocyst before vitrification: in the first group, Laser beam was used while in the second group the micro-needle was used. Ovarian stimulation was done before the ICSI procedure either by long, short or antagonist protocol.The statistical analysis of our study revealed that there was no statistically significant difference between the two groups regarding age, number of cases, AMH, Basal FSH, BMI, male factor, usage ovarian stimulation protocol, high quality blastocysts, the mean number of transferred embryos. While, there was a statistically significant difference between two groups after thawing withLASER artificial shrinkage of human blastocysts is a promising technology that could be implemented on a wider basis to improve ART practice, as our study revealed that the usage of LASER pulse for artificial shrinkage of blastocysts before vitrification has better implantation rate as well as better chemical and clinical pregnancy rate in comparison to the usage of micro-needle artificial shrinkage of blastocysts before vitrification. There is a statistically significant difference regarding live birth rate being more in the LASER group as compared to needle group, also the number of twins ether identical or non-identical are larger in laser group than in needle group but with no statistically significant difference. |
| Databáze: | OpenAIRE |
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