Molecular epidemiology of Enterobacteriaceae producing extended-spectrum beta-lactamase in a French university-affiliated hospital
| Autor: | Patrick Plésiat, Xavier Bertrand, Didier Hocquet, Daniel Talon, K. Boisson, E. Siebor |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Microbiology (medical)
DNA Bacterial Drug resistance Biology Enterobacter aerogenes beta-Lactamases Microbiology Hospitals University Enterobacteriaceae Intensive care Drug Resistance Bacterial polycyclic compounds Pulsed-field gel electrophoresis Escherichia coli Humans Pharmacology (medical) Escherichia coli Infections Antibacterial agent Retrospective Studies Molecular Epidemiology Molecular epidemiology Enterobacteriaceae Infections General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Electrophoresis Gel Pulsed-Field Klebsiella Infections Klebsiella pneumoniae Infectious Diseases bacteria France Pfge analysis |
| Zdroj: | International journal of antimicrobial agents. 22(2) |
| ISSN: | 0924-8579 |
| Popis: | We conducted a retrospective study to investigate the epidemiology of Enterobacteriaceae producing extended-spectrum beta-lactamase (ESBLE) in our hospital. We determined the occurrence of extended-spectrum beta-lactamase (ESBL) in Enterobacteriaceae over a 2-year period. We also characterised ESBLs by isoelectric focusing (IEF) and investigated the epidemiological relatedness of EBLSE by pulsed field gel electrophoresis (PFGE). During this period, 70 patients were colonised/infected with one or several strains of EBLSE, giving a crude incidence of 0.095 per 1000 patient-days. We found that ESBL-producing Enterobacter aerogenes were the main source of ESBLE dissemination. Indeed, 59.5% of ESBLE were E. aerogenes and 21.9% of the other ESBLE resulted from a plasmid transfer originating from E. aerogenes. IEF and PFGE analysis demonstrated that the dissemination of ESBL from E. aerogenes in our hospital was due to a single clone that always harbours TEM-24. This emphasises the importance of standard contact isolation precautions and the early detection of ESBLE-colonised patients in high risk departments like intensive care units. |
| Databáze: | OpenAIRE |
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