Cetylpyridinium chloride (CPC) reduces zebrafish mortality from influenza infection: Super-resolution microscopy reveals CPC interference with multiple protein interactions with phosphatidylinositol 4,5-bisphosphate in immune function
| Autor: | Marissa S. Kinney, John E. Burnell, Christian M. Potts, Sasha R. Weller, Bailey E. West, Prakash Raut, Brandy L. Soos, Julie A. Gosse, Benjamin L. King, Suraj Sangroula, Bright Obeng, Samuel T. Hess |
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| Rok vydání: | 2022 |
| Předmět: |
Viral protein
Hemagglutinin (influenza) Cetylpyridinium macromolecular substances Cell Communication medicine.disease_cause Phosphatidylinositols Toxicology chemistry.chemical_compound Mice Influenza Human medicine Animals Humans Phosphatidylinositol MARCKS Pandemics Zebrafish Antibacterial agent Mammals Pharmacology biology Chemistry SARS-CoV-2 technology industry and agriculture Degranulation Immunity COVID-19 Cell biology Rats Pleckstrin homology domain Phosphatidylinositol 4 5-bisphosphate Microscopy Fluorescence biology.protein Dinucleoside Phosphates |
| Zdroj: | Toxicology and Applied Pharmacology. 440:115913 |
| ISSN: | 0041-008X |
| Popis: | The COVID-19 pandemic raises significance for a potential influenza therapeutic compound, cetylpyridinium chloride (CPC), which has been extensively used in personal care products as a positively-charged quaternary ammonium antibacterial agent. CPC is currently in clinical trials to assess its effects on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) morbidity. Two published studies have provided mouse and human data indicating that CPC may alleviate influenza infection, and here we show that CPC (0.1 μM, 1 hour) reduces zebrafish mortality and viral load following influenza infection. However, CPC mechanisms of action upon viral-host cell interaction are currently unknown. We have utilized super-resolution fluorescence photoactivation localization microscopy to probe the mode of CPC action. Reduction in density of influenza viral protein hemagglutinin (HA) clusters is known to reduce influenza infectivity: here, we show that CPC (at non-cytotoxic doses, 5-10 µM) reduces HA density and number of HA molecules per cluster within the plasma membrane of NIH-3T3 mouse fibroblasts. HA is known to colocalize with the negatively-charged mammalian lipid phosphatidylinositol 4,5-bisphosphate (PIP2); here, we show that nanoscale co-localization of HA with the PIP2-binding Pleckstrin homology (PH) reporter in the plasma membrane is diminished by CPC. CPC also dramatically displaces the PIP2-binding protein myristoylated alanine-rich C-kinase substrate (MARCKS) from the plasma membrane of rat RBL-2H3 mast cells; this disruption of PIP2 is correlated with inhibition of mast cell degranulation. Together, these findings offer a PIP2-focused mechanism underlying CPC disruption of influenza and suggest potential pharmacological use of this drug as an influenza therapeutic to reduce global deaths from viral disease. |
| Databáze: | OpenAIRE |
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