Hypercapnia potentiates HIF-1α activation in the brain of rats exposed to intermittent hypoxia
| Autor: | V. P. Kulikov, Pavel D. Kovzelev, Natalia A. Malinovskaya, Andrey Morgun, P. P. Tregub, Elena D. Osipova, Dmitry A. Kuzovkov |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Physiology Hippocampal formation In Vitro Techniques Neuroprotection Hippocampus Hypercapnia Internal medicine medicine Animals Respiratory system Hypoxia Transcription factor Cerebral Cortex Chemistry General Neuroscience Brain Intermittent hypoxia Hypoxia (medical) Hypoxia-Inducible Factor 1 alpha Subunit respiratory tract diseases Rats medicine.anatomical_structure Endocrinology Cerebral cortex Astrocytes medicine.symptom circulatory and respiratory physiology Signal Transduction |
| Zdroj: | Respiratory physiologyneurobiology. 278 |
| ISSN: | 1878-1519 |
| Popis: | The mechanisms and signalling pathways of the neuroprotective effect of hypercapnia and its combination with hypoxia are poorly understood. The study aims to test the hypothesis about the potentiating effect of hypercapnia on hypoxia adaptation systems directly related to hypoxia-induced factor 1α (HIF-1α). In this study we assessed HIF-1α content in hippocampal extracts and astrocytes obtained from Wistar male rats exposed to different respiratory conditions (7- or 15-fold of hypoxia and/or hypercapnia). In addition, HIF-1α content in astrocytes was assessed in in vitro model of chemical hypoxia as well as in the cerebral cortex after photothrombotic damage of this brain region. This study indicates increased levels of HIF1α in hippocampal extracts, astrocytes, and in cells of the near-stroke region of the cerebral cortex in rats exposed to hypoxia and hypercapnic hypoxia, but not hypercapnia alone. In in vitro study, hypercapnia facilitates the effects of acute chemical hypoxia observed in astrocytes. Thus, hypercapnia does not increase the level of transcription factor HIF-1α. However, the combined effects of hypercapnia and hypoxia in in vitro simulations of acute chemical hypoxia potentiate the accumulation of HIF-1α. |
| Databáze: | OpenAIRE |
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