Secretion of listeriolysin by Brucella suis inhibits its intramacrophagic replication
| Autor: | Aroem Naroeni, Ivaylo Gentschev, Jean-Pierre Liautard, Stephan Köhler, Marion Layssac, Michael Rittig |
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| Rok vydání: | 2001 |
| Předmět: |
Immunology
Bacterial Toxins Biology Hemolysin Proteins medicine.disease_cause Microbiology Listeria monocytogenes Phagosomes medicine Animals Humans Secretion Heat-Shock Proteins Phagosome Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Macrophages Hemolysin biology.organism_classification Brucella Microscopy Electron Infectious Diseases Brucella suis Parasitology Cytolysin Rabbits Brucella melitensis |
| Zdroj: | Infection and immunity. 69(4) |
| ISSN: | 0019-9567 |
| Popis: | The introduction into Brucella suis 1330 of a plasmid allowing the heterologous expression of a hybrid cytolysin containing listeriolysin from Listeria monocytogenes , and its export via the Escherichia coli hemolysin secretion pathway, resulted in secretion of active listeriolysin monitored by erythrocyte lysis. In contrast to observations with the nonhemolytic control strain, the phagosomes of infected human monocytes containing the hemolytic B. suis were partially disrupted, and this strain failed to multiply in human macrophage-like cells. These results added strong evidence supporting the proposal that the phagosome of the macrophage was the predominant niche of brucellae in their mammalian hosts. |
| Databáze: | OpenAIRE |
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