Protective effect of wogonin on inflammatory responses in BisGMA-treated macrophages through the inhibition of MAPK and NFκB pathways
| Autor: | Li‐Chiu Yang, Yu‐Chao Chang, Chen‐Yu Chiang, Fu‐Mei Huang, Ni‐Yu Su, Yu‐Hsiang Kuan |
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| Rok vydání: | 2022 |
| Předmět: |
Lipopolysaccharides
Tumor Necrosis Factor-alpha Health Toxicology and Mutagenesis Macrophages NF-kappa B Anti-Inflammatory Agents Nitric Oxide Synthase Type II General Medicine Management Monitoring Policy and Law Toxicology Nitric Oxide Cyclooxygenase 2 Cytokines Phosphorylation Extracellular Signal-Regulated MAP Kinases |
| Zdroj: | Environmental toxicologyREFERENCES. 37(12) |
| ISSN: | 1522-7278 |
| Popis: | Composites, resins, and sealants that are commonly used in orthopedics and dentistry are based on 2,2-bis[p-(2'-hydroxy-3'-methacryloxypropoxy)phenylene]propane (BisGMA), which induces proinflammatory responses in macrophages. The present study aimed to explore the anti-inflammatory responses of wogonin, which is a natural dihydroxyl flavonoid compound, in BisGMA-treated macrophages. According to the findings, wogonin exhibits anti-inflammatory, antiallergic, anticancer, and antioxidative properties. The generation of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) were noted to be inhibited by wogonin in BisGMA-treated macrophages. Furthermore, the production of proinflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 was reduced. In addition, BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation and inhibitor of κB (IκB) degradation were inhibited. Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited. All these effects were induced by wogonin in the macrophages in a concentration-dependent manner. Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB. These results indicated that rutin is a potential anti-inflammatory agent for BisGMA-treated macrophages that undergo NFκB p65 phosphorylation and IκB degradation through upstream MAPK phosphorylation. Therefore, wogonin inhibits BisGMA-induced proinflammatory responses in macrophages through the regulation of the NFκB pathway and its upstream factor, MAPK. |
| Databáze: | OpenAIRE |
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