The effect of thrombospondin on oral squamous carcinoma cell invasion of collagen
| Autor: | Thomas N. Wang, Mark S. Granick, George P. Tuszynski, Vicki L. Rothman, Mark P. Solomon, Xiao-hua Qian |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
CD36 Antigens
endocrine system Pathology medicine.medical_specialty Integrins Cell Count Culture Media Serum-Free Extracellular matrix immune system diseases Transforming Growth Factor beta medicine Tumor Cells Cultured Humans Microscopy Phase-Contrast Neoplasm Invasiveness Amino Acid Sequence Receptor Thrombospondin Membrane Glycoproteins Polycarboxylate Cement biology Dose-Response Relationship Drug Cell growth business.industry virus diseases Antibodies Monoclonal Membranes Artificial General Medicine Squamous carcinoma Tumor progression Immunoglobulin G biology.protein Cancer research Carcinoma Squamous Cell Disease Progression Diffusion Chambers Culture Surgery Mouth Neoplasms Collagen Antibody business Thrombospondins Cell Adhesion Molecules Transforming growth factor |
| Zdroj: | American journal of surgery. 170(5) |
| ISSN: | 0002-9610 |
| Popis: | Background: Thrombospondin (TSP), a cell matrix protein, and transforming growth factor s (TGF-s), a growth regulatory protein, play roles in tumor progression. The purpose of this study was to investigate the effects of TSP and TGF-s on tumor cell invasion. Materials and methods: Tumor cell invasion assays were performed using a modified Boyden chamber apparatus with collagen-coated membranes. The KB oral carcinoma cell line was studied in serum-free media. Invasion was measured as the summation of the number of cells in five representative low-power fields (X 100) traversing the collagen barrier after a 3-hour incubation period. The effects of antibodies against TSP, TGF-s and the cysteineserine-valine-threonine-cysteine-glycine (CSVTCG)-specific TSP receptor were also evaluated. Results: TSP caused a dose-dependent stimulation of tumor cell invasion. Antibodies against TSP, its CSVTCG-specific receptor, and TGF-s inhibited TSP-promoted invasion by 50% to 71%. Conclusions: TSP and its CSVTCG-specific receptor promote KB cell invasion of collagen through the production and/or activation of TGF-s. |
| Databáze: | OpenAIRE |
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