Effect of Long-Term Retention of Gadolinium on Metabolism of Deep Cerebellar Nuclei After Repeated Injections of Gadodiamide in Rats
| Autor: | Michael R. Sperling, Cécile Factor, Rebecca Buchholz, Stefanie Fingerhut, Dounia El Hamrani, Uwe Karst, Philippe Robert, William Même, Sandra Même, Véronique Vives |
|---|---|
| Přispěvatelé: | Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC) |
| Rok vydání: | 2019 |
| Předmět: |
Gadolinium DTPA
Pathology medicine.medical_specialty Cerebellum gadolinium retention Magnetic Resonance Spectroscopy cerebellum [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging Gadolinium chemistry.chemical_element Contrast Media Deep cerebellar nuclei 030218 nuclear medicine & medical imaging Time Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine La icp ms medicine Animals Radiology Nuclear Medicine and imaging LA-ICP-MS Long term retention Gadodiamide deep cerebellar nuclei General Medicine Metabolism Rats Sprague dawley medicine.anatomical_structure chemistry Cerebellar Nuclei Models Animal Female 1 H-MRS 030217 neurology & neurosurgery medicine.drug MRI |
| Zdroj: | Investigative Radiology Investigative Radiology, Lippincott, Williams & Wilkins, 2020, 55 (2), pp.120-128. ⟨10.1097/RLI.0000000000000621⟩ |
| ISSN: | 1536-0210 0020-9996 |
| DOI: | 10.1097/RLI.0000000000000621⟩ |
| Popis: | International audience; OBJECTIVES:The aim of this study was to determine potential metabolism and histological modifications due to gadolinium retention within deep cerebellar nuclei (DCN) after linear gadolinium-based contrast agent injection (gadodiamide) in rats at 1 year after the last injection.MATERIALS AND METHODS:Twenty female rats received 20 doses of gadodiamide (0.6 mmol of gadolinium per kilogram each) over 5 weeks. They were followed at 1 week (M0), 6 weeks (M1), and 54 to 55 weeks (M13) postinjections to evaluate hypersignal on unenhanced T1-weighted magnetic resonance imaging and metabolic alterations by H magnetic resonance spectroscopy (H-MRS). At 1 year postinjections, brains were sampled to determine the localization of gadolinium within cerebellum by laser ablation inductively coupled mass spectroscopy and to evaluate morphological changes by semiquantitative immunofluorescence analysis.RESULTS:There is a significant increase of the ratio DCN/brainstem for the gadodiamide group at M0 (+7.2% vs control group = 0.989 ± 0.01), M1 (+7.6% vs control group = 1.002 ± 0.018), and it lasted up to M13 (+4.7% vs control group = 0.9862 ± 0.008). No variation among metabolic markers (cellular homeostasis [creatine, choline, taurine], excitatory neurotransmitter [glutamate], and metabolites specific to a cellular compartment [N-acetyl aspartate for neurons and myo-inositol for glial cells]) were detected by H-MRS between gadodiamide and saline groups at M0, M1, and M13. At M13, laser ablation inductively coupled mass spectroscopy demonstrated that long-term gadolinium retention occurred preferentially in DCN. No histological abnormalities (including analysis of astrocytes, neurons, and microglial cells) were found in the rostral part of DCN.CONCLUSIONS:Repeated administration of gadodiamide lead to a retention of gadolinium preferentially within DCN at 1 year postinjections. This retention did not lead to any detectable changes of the measured metabolic biomarkers nor histological alterations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|