In silico characterisation and functional validation of chilling tolerant divergence 1 (COLD1) gene in monocots during abiotic stress

Autor:	T S Sarath Padmanabhan, V. M. Manoj, Chinnaswamy Appunu, Ramalingam Sathishkumar, S. Dhivya, J. Ashwin Narayan, Pushpanathan Anunanthini
Rok vydání:	2019
Předmět:	0106 biological sciences 0301 basic medicine In silico Plant Science Oryza 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Stress Physiological Abscisic acid Gene Plant Proteins G protein-coupled receptor Regulation of gene expression Genetics Oryza sativa biology Abiotic stress food and beverages biology.organism_classification Droughts 030104 developmental biology chemistry Agronomy and Crop Science 010606 plant biology & botany
Zdroj:	Functional Plant Biology. 46:524
ISSN:	1445-4408
DOI:	10.1071/fp18189
Popis:	The G protein-coupled receptor is one of the major transmembrane proteins in plants. It consists of an α subunit, a β subunit and three γ subunits. Chilling tolerant divergence 1 (COLD1) includes a Golgi pH receptor (GPHR) domain, which maintains cell membrane organisation and dynamics, along with abscisic acid linked G protein-coupled receptor (ABA_GPCR) that regulates the signalling pathways during cold stress. In the present study, we performed characterisation of a homologous COLD1 from the economically important monocot species Oryza sativa L., Zea mays L., Sorghum bicolor (L.)Moench and Erianthus arundinaceus (L.) Beauv. IK 76-81, a wild relative of Saccharum. COLD1 was isolated from E. arundinaceus IK 76-81, analysed for its evolution, domain, membrane topology, followed by prediction of secondary, tertiary structures and functionally validated in all four different monocots. Gene expression studies of COLD1 revealed differential expression under heat, drought, salinity and cold stresses in selected monocots. This is the first study on regulation of native COLD1 during abiotic stress in monocots, which has opened up new leads for trait improvement strategies in this economically important crop species.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6641b6ed08c1b257d1d96a7c23aea56 https://doi.org/10.1071/fp18189 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.