ZEN-4/MKLP1 Is Required to Polarize the Foregut Epithelium
| Autor: | Jennifer R. Saam, Susan E. Mango, Michael F. Portereiko |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Green Fluorescent Proteins
Kinesins Biology Microtubules Models Biological Polymorphism Single Nucleotide General Biochemistry Genetics and Molecular Biology Epithelium 03 medical and health sciences 0302 clinical medicine Transformation Genetic Cell cortex Animals Caenorhabditis elegans Caenorhabditis elegans Proteins Mitosis Actin Protein Kinase C 030304 developmental biology DNA Primers 0303 health sciences Agricultural and Biological Sciences(all) Cadherin Biochemistry Genetics and Molecular Biology(all) Spindle midzone GTPase-Activating Proteins fungi Temperature Cell Polarity Apical membrane Cadherins Immunohistochemistry Cell biology Luminescent Proteins Centralspindlin Mutation General Agricultural and Biological Sciences Digestive System 030217 neurology & neurosurgery Cytokinesis |
| Zdroj: | Current Biology. 14(11):932-941 |
| ISSN: | 0960-9822 |
| DOI: | 10.1016/j.cub.2004.05.052 |
| Popis: | Background: Epithelial tubes are a key component of organs and are generated from cells with distinct apico-basolateral polarity. Here, we describe a novel function during tubulogenesis for ZEN-4, the Caenorhabditis elegans ortholog of mitotic kinesin-like protein 1 (MKLP1), and CYK-4, which contains a RhoGAP (GTPase-activating protein) domain. Previous studies revealed that these proteins comprise centralspindlin (a complex that functions during mitosis to bundle microtubules), construct the spindle midzone, and complete cytokinesis. Results: Our analyses demonstrate that ZEN-4/MKLP1 functions postmitotically to establish the foregut epithelium. Mutants that lack ZEN-4/MKLP1 express polarity markers but fail to target these proteins appropriately to the cell cortex. Affected proteins include PAR-3/Bazooka and PKC-3/atypical protein kinase C at the apical membrane domain, and HMR-1/cadherin and AJM-1 within C. elegans apical junctions (CeAJ). Microtubules and actin are disorganized in zen-4 mutants compared to the wild-type. Conclusion: We suggest that ZEN-4/MKLP1 and CYK-4/RhoGAP regulate an early step in epithelial polarization that is required to establish the apical domain and CeAJ. |
| Databáze: | OpenAIRE |
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