Docking of Antibodies into the Cavities of DNA Origami Structures
| Autor: | Chunhai Fan, Mattia De Stefano, Christian B. Rosen, Xiangyuan Ouyang, Abhichart Krissanaprasit, Sarah Helmig, Tianqiang Liu, Anne Louise Bank Kodal, Kurt V. Gothelf |
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| Rok vydání: | 2017 |
| Předmět: |
Tris
Nanostructure Lysine Nanotechnology 02 engineering and technology Microscopy Atomic Force 010402 general chemistry 01 natural sciences Antibodies Catalysis chemistry.chemical_compound Microscopy Electron Transmission DNA origami Histidine General Medicine General Chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Docking (molecular) Covalent bond Immunoglobulin G DNA Viral Biophysics Nucleic Acid Conformation Binding Sites Antibody Self-assembly 0210 nano-technology Bacteriophage M13 |
| Zdroj: | Angewandte Chemie International Edition. 56:14423-14427 |
| ISSN: | 1433-7851 |
| DOI: | 10.1002/anie.201706765 |
| Popis: | Immobilized antibodies are extensively employed for medical diagnostics, such as in enzyme-linked immunosorbent assays. Despite their widespread use, the ability to control the orientation of immobilized antibodies on surfaces is very limited. Herein, we report a method for the covalent and orientation-selective immobilization of antibodies in designed cavities in 2D and 3D DNA origami structures. Two tris(NTA)-modified strands are inserted into the cavity to form NTA-metal complexes with histidine clusters on the Fc domain. Subsequent covalent linkage to the antibody was achieved by coupling to lysine residues. Atomic force microscopy (AFM) and transmission electron microscopy (TEM) confirmed the efficient immobilization of the antibodies in the origami structures. This increased control over the orientation of antibodies in nanostructures and on surfaces has the potential to direct the interactions between antibodies and targets and to provide more regular surface assemblies of antibodies. |
| Databáze: | OpenAIRE |
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