Conventional and real time RT-PCR assays for the detection and differentiation of variant rabbit hemorrhagic disease virus (RHDVb) and its recombinants
| Autor: | Francisco Parra, Kevin P. Dalton, G. Chacón, J.M. Martín Alonso, A.A. Benito, J.L. Arnal |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Hemorrhagic Disease Virus Rabbit Biology Polymerase Chain Reaction Genome Virus DNA sequencing law.invention 03 medical and health sciences law Virology Animals Coding region Polymerase chain reaction Caliciviridae Infections Recombination Genetic Genetics Genetic Variation Amplicon humanities 030104 developmental biology Real-time polymerase chain reaction Spain Recombinant DNA Rabbits |
| Zdroj: | Journal of Virological Methods. 251:118-122 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/j.jviromet.2017.10.009 |
| Popis: | Since its emergence, variant RHDV (RHDVb/RHDV2) has spread throughout the Iberian Peninsula aided by the apparent lack of cross protection provided by classic (genogroup 1; G1) strain derived vaccines. In addition to RHDVb, full-length genome sequencing of RHDV strains has recently revealed the circulation of recombinant viruses on the Iberian Peninsula. These recombinant viruses contain the RHDVb structural protein encoding sequences and the non-structural coding regions of either pathogenic RHDV-G1 strains or non-pathogenic (np) rabbit caliciviruses. The aim of the work was twofold: firstly to validate a diagnostic real time RT-PCR developed in 2012 for the detection of RHDVb strains and secondly, to design a conventional RT-PCR for the differentiation of RHDVb strains from RHDVb recombinants by subsequent sequencing of the amplicon. |
| Databáze: | OpenAIRE |
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