Impacts of bisphenol A analogues on zebrafish post-embryonic brain

Autor: Pascal Coumailleau, Thierry Charlier, Elisabeth Pellegrini, Sarah Trempont
Přispěvatelé: Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), 7CQ434-S0, Défis Emergents 2018, 15HC434-03D, La région Bretagne (SAD), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )
Jazyk: angličtina
Rok vydání: 2020
Předmět:
aromatase
medicine.medical_specialty
endocrine system
Embryo
Nonmammalian
animal structures
Bisphenol
Endocrinology
Diabetes and Metabolism
[SDV]Life Sciences [q-bio]
Embryonic Development
030209 endocrinology & metabolism
Endocrine Disruptors
Bisphenol AF
Animals
Genetically Modified
03 medical and health sciences
Cellular and Molecular Neuroscience
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Phenols
bisphenol
Internal medicine
medicine
Animals
Sulfones
Aromatase
Benzhydryl Compounds
hypothalamus
Zebrafish
cyp19a1b
biology
Endocrine and Autonomic Systems
urogenital system
fungi
Brain
Embryo
biology.organism_classification
Radial glial cell
3. Good health
Preoptic area
neurogenesis
medicine.anatomical_structure
chemistry
Hypothalamus
Larva
biology.protein
030217 neurology & neurosurgery
hormones
hormone substitutes
and hormone antagonists
Zdroj: Journal of Neuroendocrinology
Journal of Neuroendocrinology, 2020, 32 (8), pp.e12879. ⟨10.1111/jne.12879⟩
Journal of Neuroendocrinology, Wiley, 2020, 32 (8), pp.e12879. ⟨10.1111/jne.12879⟩
ISSN: 0953-8194
1365-2826
Popis: International audience; Bisphenol A (BPA) is a widely studied and well-recognised endocrine-disrupting chemical, and one of the current issues is its safe replacement by various analogues. Using larva zebrafish as a model, the present study reveals that moderate and chronic exposure to BPA analogues such as bisphenol S, bisphenol F and bisphenol AF may also affect vertebrate neurodevelopment and locomotor activity. Several parameters of embryo-larval development were investigated, such as mortality, hatching, number of mitotically active cell, as defined by 5-bromo-2'-deoxyuridine incorporation and proliferative cell nuclear antigen labelling, aromatase B protein expression in radial glial cell and locomotor activity. Our results show that exposure to several bisphenol analogues induced an acceleration of embryo hatching rate. At the level of the developing brain, a strong up-regulation of the oestrogen-sensitive Aromatase B was also detected in the hypothalamic region. This up-regulation was not associated with effects on the numbers of mitotically active progenitors nor differentiated neurones in the preoptic area and in the nuclear recessus posterior of the hypothalamus zebrafish larvae. Furthermore, using a high-throughput video tracking system to monitor locomotor activity in zebrafish larvae, we show that some bisphenol analogues, such as bisphenol AF, significantly reduced locomotor activity following 6 days of exposure. Taken together, our study provides evidence that BPA analogues can also affect the neurobehavioural development of zebrafish.
Databáze: OpenAIRE
Externí odkaz: