Gene Transfer with Echo-enhanced Contrast Agents: Comparison between Albunex, Optison, and Levovist in Mice—Initial Results
| Autor: | Masahide Kuroki, Katsuro Tachibana, Tieli Li, Motomu Kuroki |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
DNA
Bacterial Pathology medicine.medical_specialty medicine.medical_treatment Genetic Vectors Green Fluorescent Proteins Contrast Media CHO Cells Injections Intramuscular Green fluorescent protein Mice Cricetulus Polysaccharides Albumins Cricetinae medicine Animals Radiology Nuclear Medicine and imaging Fluorocarbons Mice Inbred BALB C Microbubbles Therapeutic ultrasound business.industry Ultrasound Genetic transfer Gene Transfer Techniques Albumin Transfection Molecular biology Luminescent Proteins Female business Intramuscular injection Plasmids |
| Zdroj: | Radiology. 229:423-428 |
| ISSN: | 1527-1315 0033-8419 |
| DOI: | 10.1148/radiol.2292020500 |
| Popis: | To determine if commercially available echo-enhanced microbubble contrast agents could be used to increase gene transfection efficiency by means of relatively low-intensity ultrasound-mediated microbubble destruction in skeletal muscles.Three types of ultrasound microbubble contrast agents (0.01 mL of albumin [Albunex] and human albumin [Optison] and 10 mg/mL of SH U 508A [Levovist]) were each separately mixed with the reporter plasmid DNA (25 microg) encoding green fluorescent protein (GFP) prior to intramuscular injection into the quadriceps muscle of a mouse thigh bilaterally (seven mice per contrast agent). One of the muscle sites that was injected with plasmid DNA was irradiated with low-intensity therapeutic ultrasound (1 MHz) at an intensity of 2.0 W/cm2 for 2 minutes. Mice were sacrificed 7 days after ultrasound treatment for gene expression assay. The number of GFP-expressing muscle fibers was counted. Statistical significance was determined with a two-tailed Student t test. P.05 was considered to indicate statistically significant difference.Muscle tissue exposed to ultrasound with air-filled Albunex or Levovist microbubbles revealed no difference in the number of GFP-expressing muscle fibers compared with the control non-ultrasound-exposed muscle. Albumin-coated octafluoropropane gas-filled Optison microbubbles showed a 10-fold increase in the number of GFP-expressing fibers (P.05).Low-intensity ultrasound with echo-enhanced Optison induced efficient gene transfer unlike that with Albunex or Levovist. |
| Databáze: | OpenAIRE |
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