The plasmidome of a Salmonella enterica serovar Derby isolated from pork meat
| Autor: | Gerhard Schöfl, Hans Peter Saluz, Anne Bleicher, M. R. Rodicio |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
DNA Replication
DNA Bacterial Salmonella Meat Swine Biology medicine.disease_cause Integron Synteny Microbiology Nalidixic Acid Plasmid Drug Resistance Multiple Bacterial medicine Animals Replicon Molecular Biology Escherichia coli Phylogeny Genetics Base Sequence Plasmid partitioning Salmonella enterica Molecular Sequence Annotation Chromosomes Bacterial biology.organism_classification Genes Bacterial Conjugation Genetic Food Microbiology biology.protein Plasmidome Plasmids |
| Zdroj: | Plasmid. 69:202-210 |
| ISSN: | 0147-619X |
| DOI: | 10.1016/j.plasmid.2013.01.001 |
| Popis: | The complete nucleotide sequences of four plasmids hosted by a Salmonella enterica serovar. Derby strain 6MK1 isolated from pork were determined by shotgun Sanger sequencing. A 107,637 base pairs (bp) conjugative plasmid pSD107 containing 150 putative coding sequences (CDS) could be assigned to the narrow host range incompatibility group IncI1. A detailed annotation of all CDS was carried out, revealing the presence of genes needed for plasmid replication, conjugal transfer, plasmid partitioning and stability as well as resistance to antimicrobials. The resistance determinants dhfrA1, aadA1, qacEΔ1, sul1 (supplied by a class 1 integron) , bla TEM−1b (carried by a truncated Tn 2 flanked by IS 26 ), sul2 and strAB confer multidrug resistance to the host bacterium. In addition to pSD107, three small cryptic plasmids pSD4.0, pSD4.6 and pSD5.6 were identified, showing significant sequence similarities to already known replicons of Escherichia coli and S. enterica . In conjugation experiments performed on solid medium, pSD107 was successfully transferred to a nalidixic acid resistant E. coli DH5α, mobilizing pSD4.0 and, more infrequently, also pSD4.6. All transferred plasmids were stably propagated in the recipient strain without selective pressure for approximately 66 generations. The absolute plasmid copy numbers were determined in real time PCR experiments, revealing an approximate 1:1:1:1 ratio of the four replicons compared to the chromosome. The evolutionary position of pSD107 within the IncI1 family of plasmids was inferred from a maximum likelihood phylogenetic tree and by comparison of genetic key elements in a set of 17 IncI1 reference plasmids. |
| Databáze: | OpenAIRE |
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