Cellular localisation of HHV-8 in Castleman's disease: is there a link with lymph node vascularity?
| Autor: | M O'Donovan, K C Gatter, J Russell, C Kenny, John J. O'Leary, N Bermingham, Simon Biddolph, Orla Sheils, K Luttich, M Ring, Christopher Martin, M M Kennedy, Mary Rose Sweeney, V Uhlmann, I Silva, S Picton, Sebastian Lucas, D D Howells |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
CD31
Paper Adult Male Pathology medicine.medical_specialty viruses CD34 Antigens CD34 Spindle Apparatus Biology Polymerase Chain Reaction Pathology and Forensic Medicine Open Reading Frames Vascularity immune system diseases hemic and lymphatic diseases Biopsy medicine Humans Lymph node Kaposi's sarcoma In Situ Hybridization B-Lymphocytes Factor VIII Paraffin Embedding medicine.diagnostic_test Castleman Disease virus diseases Middle Aged medicine.disease Immunohistochemistry Platelet Endothelial Cell Adhesion Molecule-1 medicine.anatomical_structure Herpesvirus 8 Human Female Lymph Sarcoma Lymph Nodes medicine.symptom Endothelium Lymphatic |
| Popis: | Aims—Human herpesvirus 8 (HHV-8) has been identified in multicentric Castleman's disease and in angioimmunoblastic lymphadenopathies. However, the presence of the virus does not necessarily indicate an aetiological role in these conditions. This study investigates the cell types infected by HHV-8 in Castleman's disease and examines the correlation between HHV-8 and Castleman's disease lymph node angiogenesis. Methods—Sixteen formalin fixed, paraffin wax embedded samples from patients with Castleman's disease (six multicentric, 10 solitary) were examined for the presence of HHV-8 using the polymerase chain reaction (PCR), non-isotopic in situ hybridisation, PCR in situ hybridisation (PCR-ISH), and real time quantitative TaqMan PCR to HHV-8 open reading frame 26 (ORF-26), and viral (v)-cyclin encoding regions. Vascularity was assessed using CD34, CD31, and factor VIII immunocytochemistry, and lymph nodes were scored as “low” or “high”. Results—Five multicentric Castleman's disease and two solitary Castleman's disease biopsies were positive for HHV-8. HHV-8 was identified in approximately 10% of intranodal B lymphocytes, in endothelial cells, and in subcapsular spindle cell proliferations. The copy number of HHV-8 was low at 10–50 copies/1000 cells. The highest copy number was in subcapsular spindle cells. There was no correlation between vascularity score and HHV-8 status. Conclusion—The preferential localisation of HHV-8 in subcapsular spindle cell proliferations (where early intranodal Kaposi's sarcoma initiates) and endothelial cells in Castleman's disease might finally explain the link between intranodal Kaposi's sarcoma and Castleman's disease. |
| Databáze: | OpenAIRE |
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