Mechanically induced orientation of adult rat cardiac myocytes in vitro
| Autor: | J.-L Samuel, Herman H. Vandenburgh |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Time Factors
Cell division Clinical Biochemistry Population Silicones Stimulation Plant Science Biology Cell Adhesion Animals Myocyte Cytoskeleton Cell adhesion education Cells Cultured education.field_of_study Myocardium Rats Inbred Strains Cell Biology Adhesion Anatomy Rats Phenotype Cell culture Silicone Elastomers Biophysics Laminin Stress Mechanical Cell Division Biotechnology Developmental Biology |
| Zdroj: | In Vitro Cellular & Developmental Biology. 26:905-914 |
| ISSN: | 1475-2689 0883-8364 |
| DOI: | 10.1007/bf02624616 |
| Popis: | A population of freshly isolated adult rat cardiac myocytes is spatially oriented using a computerized mechanical cell stimulator device for tissue cultured cells. A continuous unidirectional stretch of the substratum at 60 to 400 micron/min for 120 to 30 min, respectively, during the cell attachment period in serum-free medium induces a significant three-fold increase in the number of rod-shaped myocytes oriented parallel to the direction of movement. The myocytes orient less well with unidirectional substratum stretching after their adhesion to the substratum. In contrast, adult myocytes plated onto a substratum undergoing continuous 10% stretch-relaxation cycling show no significant change in myocyte orientation or cytoskeletal organization. Orientation of rod-shaped myocytes is dependent on several factors other than the type of mechanical activity. These include: a) the speed of substratum movement; b) the final stretch amplitude; and c) the timing between initiation of substratum stretching and adhesion of myocytes to the substratum. Oriented adult rod shaped myocytes representing 65 to 70% of the total myocyte population in this model system can now be submitted to different patterns of repetitive mechanical stimulation for the study of stretch-induced alterations in cell growth and gene expression. |
| Databáze: | OpenAIRE |
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