Molecular detection of human mammaglobin in cerebrospinal fluid from breast cancer patient with leptomeningeal carcinomatosis
| Autor: | Enrico Battolla, Paolo Dessanti, Bartolomeo Bacigalupo, Paola Ferro, Franco Fedeli, Cesare Capellini, Mariella Dono, Antonio Tartaglione, Silvio Roncella, Luana Benedetti, Michele Moroni |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Cancer Research Pathology medicine.medical_specialty Breast Neoplasms Diagnostic tools Breast cancer Mammaglobin Cerebrospinal fluid Cytology medicine Humans Uteroglobin RNA Messenger biology business.industry Mammaglobin A Cancer Brain medicine.disease Neoplasm Proteins Real-time polymerase chain reaction Neurology Oncology biology.protein Female Neurology (clinical) Complication business Meningeal Carcinomatosis |
| Zdroj: | Journal of neuro-oncology. 91(3) |
| ISSN: | 1573-7373 |
| Popis: | Leptomeningeal (LM) carcinomatosis is an increasing clinical complication in patients with advanced breast cancer (BC). The LM carcinomatosis diagnostic procedures rely mainly on cerebrospinal fluid (CSF) cytology, although both the amount of CSF and the number of malignant cells remain limiting factors. Therefore, efforts should be made to design new highly sensitive diagnostic tools to detect malignant cells in CSF of BC patients with LM carcinomatosis. In this study, the human Mammaglobin (hMAM) mRNA amplification by RT-PCR was employed to detect metastatic cells in CSF and thus, to diagnose LM carcinomatosis in a BC patient. Our data demonstrate that hMAM transcripts are expressed in the CSF of a BC patient with LM carcinomatosis, hence making RT-PCR for hMAM a potentially suitable test to identify occult BC cells in the brain. |
| Databáze: | OpenAIRE |
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