Enhanced fatty acid production in engineered chemolithoautotrophic bacteria using reduced sulfur compounds as energy sources
| Autor: | Peng Zhou, T. N. M. Jewell, Harry R. Beller, Jay D. Keasling, Ee-Been Goh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
ACP acyl carrier protein lcsh:Biotechnology Endocrinology Diabetes and Metabolism polymerase chain reaction Sulfide 030106 microbiology Biomedical Engineering acyl carrier protein chemistry.chemical_element Article 03 medical and health sciences chemistry.chemical_compound PCR polymerase chain reaction Affordable and Clean Energy Thioesterase lcsh:TP248.13-248.65 Bioproducts 2.2 Factors relating to the physical environment SDG 7 - Affordable and Clean Energy Aetiology Fatty acids lcsh:QH301-705.5 chemistry.chemical_classification Thiosulfate tesA ACP biology Fatty acid biology.organism_classification Sulfur SDG 11 - Sustainable Cities and Communities Climate Action PCR lcsh:Biology (General) chemistry Wastewater Biochemistry Chemolithoautotrophic Thiobacillus denitrificans Energy source Bacteria |
| Zdroj: | Beller, H R, Zhou, P, Jewell, T N M, Goh, E-B & Keasling, J 2016, ' Enhanced fatty acid production in engineered chemolithoautotrophic bacteria using reduced sulfur compounds as energy sources ', Metabolic Engineering Communications, vol. 3, pp. 211-215 . https://doi.org/10.1016/j.meteno.2016.07.001 Beller, HR; Zhou, P; Jewell, TNM; Goh, EB; & Keasling, JD. (2016). Enhanced fatty acid production in engineered chemolithoautotrophic bacteria using reduced sulfur compounds as energy sources. Metabolic Engineering Communications, 3, 211-215. doi: 10.1016/j.meteno.2016.07.001. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/8qj052dt Metabolic Engineering Communications, Vol 3, Iss, Pp 211-215 (2016) Metabolic Engineering Communications |
| DOI: | 10.1016/j.meteno.2016.07.001 |
| Popis: | Chemolithoautotrophic bacteria that oxidize reduced sulfur compounds, such as H2S, while fixing CO2 are an untapped source of renewable bioproducts from sulfide-laden waste, such as municipal wastewater. In this study, we report engineering of the chemolithoautotrophic bacterium Thiobacillus denitrificans to produce up to 52-fold more fatty acids than the wild-type strain when grown with thiosulfate and CO2. A modified thioesterase gene from E. coli (‘tesA) was integrated into the T. denitrificans chromosome under the control of Pkan or one of two native T. denitrificans promoters. The relative strength of the two native promoters as assessed by fatty acid production in engineered strains was very similar to that assessed by expression of the cognate genes in the wild-type strain. This proof-of-principle study suggests that engineering sulfide-oxidizing chemolithoautotrophic bacteria to overproduce fatty acid-derived products merits consideration as a technology that could simultaneously produce renewable fuels/chemicals as well as cost-effectively remediate sulfide-contaminated wastewater. Highlights • Reduced S compounds and CO2 can be feedstocks for biobased chemicals and biofuels. • Fatty acids increased 52-fold in Thiobacillus denitrificans with E. coli ‘tesA. • Native T. denitrificans promoters driving ‘tesA predictably enhanced fatty acids. |
| Databáze: | OpenAIRE |
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