Characterization of a Class II Defective Transposon Carrying Two Haloacetate Dehalogenase Genes from Delftia acidovorans Plasmid pUO1
| Autor: | Keiji Nitta, Masahiro Sota, Masataka Tsuda, Haruhiko Kawasaki, Masahiro Endo |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
DNA
Bacterial Genetics Tn3 transposon Base Sequence Ecology Hydrolases Transposon Resolvases Molecular Sequence Data Genetics and Molecular Biology Biology Sleeping Beauty transposon system Polymerase Chain Reaction Applied Microbiology and Biotechnology Molecular biology P element Composite transposon Sequence Homology Nucleic Acid Proteobacteria Simple transposon DNA Transposable Elements Haloacetate dehalogenase Transposase Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 68:2307-2315 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.68.5.2307-2315.2002 |
| Popis: | The two haloacetate dehalogenase genes, dehH1 and dehH2 , on the 65-kb plasmid pUO1 from Delftia acidovorans strain B were found to be located on transposable elements. The dehH2 gene was carried on an 8.9-kb class I composite transposon (Tn Had1 ) that was flanked by two directly repeated copies of IS 1071 , IS 1071 L and IS 1071 R. The dehH1 gene was also flanked by IS 1071 L and a truncated version of IS 1071 (IS 1071 N). Tn Had1 , dehH1 , and IS 1071 N were located on a 15.6-kb class II transposon (Tn Had2 ) whose terminal inverted repeats and res site showed high homology with those of the Tn 21 -related transposons. Tn Had2 was defective in transposition because of its lacking the transposase and resolvase genes. Tn Had2 could transpose when the Tn 21 -encoded transposase and resolvase were supplied in trans . These results demonstrated that Tn Had2 is a defective Tn 21 -related transposon carrying another class I catabolic transposon. |
| Databáze: | OpenAIRE |
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