A gene signal amplifier platform for monitoring the unfolded protein response
| Autor: | Alexander L Yang, Bhagyashree Bachhav, Sahiti D Patibandla, Carlos A. Origel Marmolejo, Laura Segatori |
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| Rok vydání: | 2020 |
| Předmět: |
Transcription
Genetic Green Fluorescent Proteins Protein Serine-Threonine Kinases Biology Endoplasmic Reticulum Chromosomes eIF-2 Kinase 03 medical and health sciences Genes Reporter Endoribonucleases Gene expression Humans Molecular Biology Gene 030304 developmental biology Regulation of gene expression 0303 health sciences Gene Expression Profiling Endoplasmic reticulum 030302 biochemistry & molecular biology HEK 293 cells Computational Biology Cell Biology Activating Transcription Factor 6 Cell biology Chromatin HEK293 Cells Protein Biosynthesis Unfolded Protein Response Unfolded protein response Signal transduction |
| Zdroj: | Nature Chemical Biology. 16:520-528 |
| ISSN: | 1552-4469 1552-4450 |
| Popis: | Gene expression in mammalian cells results from coordinated protein-driven processes guided by diverse mechanisms of regulation, including protein-protein interactions, protein localization, DNA modifications and chromatin rearrangement. Regulation of gene expression is particularly important in stress-response pathways. To address the need to monitor chromosomal gene expression generating a readily detectable signal output that recapitulates gene expression dynamics, we developed a gene signal amplifier platform that links transcriptional and post-translational regulation of a fluorescent output to the expression of a chromosomal target gene. We generated a multiplex reporter system for monitoring markers of the unfolded protein response, a complex signal transduction pathway that remodels gene expression in response to proteotoxic stress in the endoplasmic reticulum. By recapitulating the transcriptional and translational control mechanisms underlying the expression of a target gene with high sensitivity, this platform provides a technology for monitoring gene expression with superior sensitivity and dynamic resolution. |
| Databáze: | OpenAIRE |
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