Influence of parasite load on the diagnosis and occurrence of eosinophilia in alcoholic patients infected with Strongyloides stercoralis
| Autor: | Joelma Nascimento de Souza, Mônica L. S. Silva, Márcia Cristina Aquino Teixeira, Neci Matos Soares, Alex Bruno da Silva Souza, E.R. Menezes, L.G. Barbosa, L.N. Oliveira, C.M. Guimarães, M.C.M. Alves, Elizabete de Jesus Inês, Victória Maria dos S. Dias |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030231 tropical medicine Biology Parasite load Sensitivity and Specificity Parasite Load Strongyloides stercoralis Agar plate 03 medical and health sciences Feces 0302 clinical medicine Eosinophilia medicine Helminths Animals Humans General Medicine Eosinophil biology.organism_classification medicine.disease Alcoholism 030104 developmental biology medicine.anatomical_structure Strongyloidiasis Immunology Animal Science and Zoology Parasitology medicine.symptom Brazil |
| Zdroj: | Journal of helminthology. 93(1) |
| ISSN: | 1475-2697 |
| Popis: | Alcoholic patients are more susceptible toStrongyloides stercoralisinfection. The chronic use of alcohol raises the levels of endogenous corticosteroids, which regulates the development of larvae and stimulates the differentiation of rhabditiform into infective filariform larvae, thus inducing internal autoinfection. Therefore, early diagnosis is important to prevent severe strongyloidiasis. The aim of this study was to evaluate the efficacy of parasitological methods, according to the parasite load and the number of stool samples, for diagnosis ofS. stercoralisinfection, as well the peripheral blood eosinophil count in alcoholic patients. A total of 330 patients were included in this study. The diagnosis was established using three parasitological methods: agar plate culture, Baermann–Moraes method and spontaneous sedimentation. Peripheral eosinophilia was considered when the level was >600 eosinophils/mm3. The agar plate culture (APC) had the highest sensitivity (97.3%). However, the analysis of multiple samples increased the sensitivity of all parasitological methods. The sensitivities of the methods were influenced by the parasite load. When the larval number was above 10, the sensitivity of APC was 100%, while in spontaneous sedimentation the sensitivity reached 100% when the larval number was above 50. In the present study, 15.4% of alcoholic patients infected withS. stercoralis(12/78) had increased peripheral blood eosinophil count (above 600 eosinophils/mm3). For an efficient parasitological diagnosis ofS. stercoralisinfection in alcoholic patients, repeated examination by two parasitological methods must be recommended, including agar plate culture due to its higher sensitivity. Moreover,S. stercoralisinfection was associated with eosinophilia, mostly in patients excreting up to 10 larvae/g faeces. |
| Databáze: | OpenAIRE |
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