The effect of low intensity shockwave treatment (Li-SWT) on human myoblasts and mouse skeletal muscle
| Autor: | Henrik Daa Schrøder, Jeeva Sellathurai, Lars Lund, Lise K. Hansen, Karthikeyan Rajagopal, Vrisha Maduri |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Programmed cell death lcsh:Diseases of the musculoskeletal system Muscle Development High-Energy Shock Waves Andrology Neovascularization Myoblasts Mice 03 medical and health sciences 0302 clinical medicine Cardiotoxin Rheumatology Skeletal muscle regeneration Animals Humans Medicine Myocyte Orthopedics and Sports Medicine Viability assay Muscle Skeletal Cells Cultured business.industry Regeneration (biology) Vascularization Skeletal muscle Cell Differentiation Li-SWT Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Ultrasonic Waves 030220 oncology & carcinogenesis Female MYF5 Angiogenesis medicine.symptom lcsh:RC925-935 business Research Article |
| Zdroj: | BMC Musculoskeletal Disorders, Vol 18, Iss 1, Pp 1-13 (2017) BMC Musculoskeletal Disorders Hansen, L K, Schrøder, H D, Lund, L, Rajagopal, K, Maduri, V & Sellathurai, J 2017, ' The effect of low intensity shockwave treatment (Li-SWT) on human myoblasts and mouse skeletal muscle ', BMC Musculoskeletal Disorders, vol. 18, 557 . https://doi.org/10.1186/s12891-017-1879-4 |
| ISSN: | 1471-2474 |
| DOI: | 10.1186/s12891-017-1879-4 |
| Popis: | Background Transplanting myogenic cells and scaffolds for tissue engineering in skeletal muscle have shown inconsistent results. One of the limiting factors is neovascularization at the recipient site. Low intensity shockwave therapy (Li-SWT) has been linked to increased tissue regeneration and vascularization, both integral to survival and integration of transplanted cells. This study was conducted to demonstrate the response of myoblasts and skeletal muscle to Li-SWT. Method Primary isolated human myoblasts and explants were treated with low intensity shockwaves and subsequently cell viability, proliferation and differentiation were tested. Cardiotoxin induced injury was created in tibialis anterior muscles of 28 mice, and two days later, the lesions were treated with 500 impulses of Li-SWT on one of the legs. The treatment was repeated every third day of the period and ended on day 14 after cardiotoxin injection.. The animals were followed up and documented up to 21 days after cardiotoxin injury. Results Li-SWT had no significant effect on cell death, proliferation, differentiation and migration, the explants however showed decreased adhesion. In the animal experiments, qPCR studies revealed a significantly increased expression of apoptotic, angiogenic and myogenic genes; expression of Bax, Bcl2, Casp3, eNOS, Pax7, Myf5 and Met was increased in the early phase of regeneration in the Li-SWT treated hind limbs. Furthermore, a late accumulative angiogenic effect was demonstrated in the Li-SWT treated limbs by a significantly increased expression of Angpt1, eNOS, iNOS, Vegfa, and Pecam1. Conclusion Treatment was associated with an early upregulation in expression of selected apoptotic, pro-inflammatory, angiogenic and satellite cell activating genes after muscle injury. It also showed a late incremental effect on expression of pro-angiogenic genes. However, we found no changes in the number of PAX7 positive cells or blood vessel density in Li-SWT treated and control muscle. Furthermore, Li-SWT in the selected doses did not decrease survival, proliferation or differentiation of myoblasts in vitro. Electronic supplementary material The online version of this article (10.1186/s12891-017-1879-4) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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