Direct electron transfer-type four-way bioelectrocatalysis of CO2/formate and NAD+/NADH redox couples by tungsten-containing formate dehydrogenase adsorbed on gold nanoparticle-embedded mesoporous carbon electrodes modified with 4-mercaptopyridine
| Autor: | Kento Sakai, Yuki Kitazumi, Kazuyoshi Takagi, Osamu Shirai, Kenji Kano |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Inorganic chemistry
chemistry.chemical_element 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Electrochemistry Photochemistry Formate dehydrogenase 01 natural sciences Redox Formate oxidation 0104 chemical sciences lcsh:Chemistry chemistry.chemical_compound Electron transfer chemistry lcsh:Industrial electrochemistry lcsh:QD1-999 Formate 0210 nano-technology Carbon Electrochemical reduction of carbon dioxide lcsh:TP250-261 |
| Zdroj: | Electrochemistry Communications, Vol 84, Iss, Pp 75-79 (2017) |
| ISSN: | 1388-2481 |
| Popis: | Tungsten-containing formate dehydrogenase from Methylobacterium extorquens AM1 (FoDH1) catalyzes formate oxidation with NAD+. FoDH1 shows little direct communication with carbon electrodes, including mesoporous Ketjen Black-modified glassy carbon electrode (KB/GCE); however, it shows well-defined direct electron transfer (DET)-type bioelectrocatalysis of carbon dioxide reduction, formate oxidation, NAD+ reduction, and NADH oxidation on gold nanoparticle (AuNP)-embedded KB/GCE treated with 4-mercaptopyridine. Microscopic measurements reveal that the AuNPs (d=5nm) embedded on the KB surface are uniformly dispersed. Electrochemical data indicate that the pyridine moiety on the AuNPs plays important roles in facilitating the interfacial electron transfer kinetics and increasing the probability of productive orientation of FoDH1. The formal potential of the electrochemical communication site, which is most probably an ion‑sulfur cluster, is evaluated as −0.591±0.005V vs. Ag|AgCl|sat. KCl from Nernst analysis of the steady-state catalytic waves. Keywords: Gold nanoparticle, Mesoporous carbon electrode, Direct electron transfer, Formate dehydrogenase, Bioelectrocatalysis, 4-Mercaptopyridine |
| Databáze: | OpenAIRE |
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