SYK and LYN Mediate B-cell Receptor-independent Calcium-induced Apoptosis in DT-40 Lymphoma B-cells
| Autor: | Heather E. Tibbles, De-Min Zhu, Alexei O. Vassilev, Fatih M. Uckun |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Cancer Research
Lymphoma B-Cell B-cell receptor Ionophore Receptors Antigen B-Cell Syk chemistry.chemical_element Apoptosis Calcium Proto-Oncogene Proteins c-fyn chemistry.chemical_compound immune system diseases LYN Proto-Oncogene Proteins hemic and lymphatic diseases Agammaglobulinaemia Tyrosine Kinase Tumor Cells Cultured medicine Animals Syk Kinase B-Lymphocytes Enzyme Precursors Chemistry Ionomycin Intracellular Signaling Peptides and Proteins Hematology Protein-Tyrosine Kinases medicine.disease Lymphoma src-Family Kinases Oncology Lymphocyte Specific Protein Tyrosine Kinase p56(lck) Cancer research Chickens |
| Zdroj: | Leukemia & Lymphoma. 43:2165-2170 |
| ISSN: | 1029-2403 1042-8194 |
| Popis: | Here, we report that the calcium ionophore ionomycin induces a massive Ca2+-dependent apoptosis in wildtype DT-40 chicken B lymphoma cells, as well as in BTK-deficient, PLCgamma2-deficient and IP3 receptor-deficient DT-40 cells, but not in LYN- or SYK-deficient DT-40 cells. Notably, the deficiency of CSK, a negative regulator of Src-family PTK, promoted ionomycin-induced apoptosis of DT-40 cells. Reconstitution of SYK-deficient cells with wild-type SYK restored the apoptotic response of the cells to ionomycin, but the expression of FYN or LCK in LYN-deficient cells did not restore the apoptotic response of LYN-deficient cells. Taken together, our data suggests that both LYN and SYK, but not BTK, FYN or LCK, are crucial mediators of BCR-independent Ca2+-induced apoptosis in DT-40 lymphoma B cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|