Improved Preventive Effects of Combined Bioactive Compounds Present in Different Blueberry Varieties as Compared to Single Phytochemicals
Autor: | Jacob J. Briedé, Theo M. de Kok, Simone G. J. van Breda |
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Přispěvatelé: | Toxicogenomics, RS: GROW - R1 - Prevention, RS: FSE MaCSBio, RS: FPN MaCSBio, RS: FHML MaCSBio |
Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
IMPACT Cyanidin Blueberry Plants Phytochemicals lcsh:TX341-641 antioxidant capacity Biology medicine.disease_cause Article blueberry extracts MECHANISMS 03 medical and health sciences chemistry.chemical_compound In vivo Gene expression medicine Humans Food science (combinations of) phytochemicals EC50 Peonidin 030109 nutrition & dietetics Nutrition and Dietetics disease risk reduction Vitamin C gene expression profiles FRUIT CANCER RADICAL FORMATION 030104 developmental biology chemistry Gene Expression Regulation DNA-DAMAGE Caco-2 Cells Quercetin lcsh:Nutrition. Foods and food supply Oxidative stress Food Science DNA Damage |
Zdroj: | Nutrients, 11(1):61. Multidisciplinary Digital Publishing Institute (MDPI) Nutrients Volume 11 Issue 1 Nutrients, Vol 11, Iss 1, p 61 (2018) |
ISSN: | 2072-6643 |
Popis: | Blueberries contain many different phytochemicals which might be responsible for their disease preventive properties. In a previously conducted human dietary intervention study, we showed that a 4-week intervention with blueberry&ndash apple juice protected the participants against oxidative stress and modulated expression of genes involved in different genetic pathways contributing to the antioxidant response. The present study investigates the effect of different blueberry varieties (Elliot, Draper, Bluecrop, and Aurora, and the blueberry&ndash apple juice from our previous human dietary intervention study), and four different single compounds (vitamin C, peonidin, cyanidin, and quercetin) on antioxidant capacity and gene expression changes in colonic cells in vitro, and compares the outcome with the earlier in vivo findings. The results demonstrate that all blueberry varieties as well as the blueberry&ndash apple juice were more effective in reducing oxidative stress as compared to the single compounds (e.g., DNA strand break reduction: EC50: Elliot 8.3 mg/mL, Aurora and Draper 11.9 mg/mL, blueberry&ndash apple juice 12.3 mg/mL, and Bluecrop 12.7 mg/mL single compounds). In addition, the gene expression profiles (consisting of 18 selected genes from the in vivo study) induced by the blueberry varieties were more similar to the profile of the human intervention study (range 44&ndash 78%). The blueberry variety Elliot showed the strongest and most similar effects, almost 80% of gene expression modulations were similar compared to the in vivo results. From the single compounds (range 17&ndash 44%), quercetin induced the most comparable gene expression changes, i.e., 44%. This approach could be useful in agriculture for identifying crop varieties containing combinations of phytochemicals which show optimal preventive capacities. |
Databáze: | OpenAIRE |
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