The Regulatory Roles of ncRNA Rli60 in Adaptability of Listeria monocytogenes to Environmental Stress and Biofilm Formation
| Autor: | Xuepeng Cai, Kun Xie, Ma Yu, Hu Zhengxiang, Peng Yelong, Jun Qiao, Chen Cheng, Chuangfu Chen, Qingling Meng, Tian-li Liu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
030106 microbiology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Listeria monocytogenes Stress Physiological medicine Humans Overlap extension polymerase chain reaction Gene Strain (chemistry) biology Biofilm Gene Expression Regulation Bacterial General Medicine biology.organism_classification Adaptation Physiological RNA Bacterial chemistry Biofilms Brain heart infusion RNA Long Noncoding Homologous recombination Bacteria |
| Zdroj: | Current Microbiology. 73:77-83 |
| ISSN: | 1432-0991 0343-8651 |
| DOI: | 10.1007/s00284-016-1028-6 |
| Popis: | Listeria monocytogenes is a facultative anaerobic Gram-positive bacterium. It is well adapted to external environments and able to infect both humans and animals. To understand the impacts of ncRNA Rli60 on the adaptability of L. monocytogenes to environmental stresses and biofilm formation, a rli60 deletion strain of L. monocytogenes (LM-Δrli60) was constructed using splicing by overlap extension PCR (SOE-PCR) and homologous recombination and then compared it with wild-type strain L. monocytogenes EGD-e in the aspects of adaptability to environmental stresses by measuring their growth under stresses of different temperatures, and acidic, alkaline, hypertonic and alcoholic conditions, and capability of biofilm formation by using crystal violet staining, as well as the transcriptional levels of genes (gltB and gltC) related to the biofilm formation by real-time quantitative PCR (qRT-PCR). The results showed that (1) the growth of LM-Δrli60 strain was significantly slower under environmental stresses of low temperature (30 °C), high temperature (42 °C), as well as alkaline and alcoholic conditions, (2) the amount of biofilm formed by LM-Δrli60 was attenuated, and (3) the transcriptional levels of gltB and gltC genes at 24 h and 48 h in LM-Δrli60 revealed a significant reduction. Overall, the results confirmed that ncRNA Rli60 plays important roles in regulating the adaptability of L. monocytogenes to environmental stresses and biofilm formation possibly through impacting the expression of gltB and gltC genes. |
| Databáze: | OpenAIRE |
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