Periodontitis contributes to aberrant metabolism in type 2 diabetes mellitus rats by stimulating the expression of adipokines

Autor: Xi Yang, Jia Ni, J. Wu, Jian Zhang, Dongying Xuan, Dan Wang, Z. Xu, Shigao Luo
Rok vydání: 2015
Předmět:
Zdroj: Journal of Periodontal Research. 51:453-461
ISSN: 0022-3484
DOI: 10.1111/jre.12322
Popis: Background Periodontitis has been associated with type 2 diabetes mellitus. We investigated the effects of local aberrant secretion of adipokines in diabetic rats on systemic metabolism. Material and Methods Otsuka Long-Evans Tokushima Fatty (OLETF) and non-diabetic Long-Evans Tokushima Otsuka rats were used as a diabetic model and associated control, respectively. Periodontitis was induced using a silk ligature for 36 wk. Rats were grouped into OLETF with (OP+) or without (OP−) periodontitis and Long-Evans Tokushima Otsuka with (LP+) or without (LP−) periodontitis. Alveolar bone resorption and destruction were evaluated by micro-computed tomography and hematoxylin and eosin staining. After 20 wk of periodontitis induction, lipids, insulin, interleukin-1, leptin and plasminogen activator inhibitor-1 were analyzed, and mRNA expressions of NF-κB, Mark8, TLR2 and -4, IKBKB and Nampt were evaluated by quantitative polymerase chain reaction in adipose tissue. Results After ligation, OLETF rats exhibited typical periodontitis lesions with the clinical features of type 2 diabetes mellitus. When compared with the OP(−) group, the area under curve of the oral glucose tolerance test and homeostatic model assessment–insulin resistance values were significantly higher in the OP(+) group. Micro-computed tomography showed that the OP(+) group had more bone resorption than the OP(−) group. When compared with the OP(−) group, the OP(+) group also exhibited higher total cholesterol (p 0.05) and higher expression of Nampt (p < 0.05) and IKBKB (p < 0.05). The LP(+) group had a trend towards higher bone resorption when compared with the LP(−) group. Additionally, no difference in white adipose tissues or adipokines was found between LP(+) and LP(−) rats. Conclusions Chronic periodontitis can alter lipid profiles in affected rats, elevate adipose tissue expression of Nampt and affect the metabolism of adipose tissue through the NF-κB pathway to inflame diabetes.
Databáze: OpenAIRE
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