Expression of the Short Form of RON/STK in Feline Mammary Carcinoma
| Autor: | Kohei Saeki, Yolanda Millán, Calogero Trupia, Raquel Sanchez Cespedez, Silvia Guil-Luna, Selina Iussich, L. Maniscalco, Francesca Gattino, Juana Martín de las Mulas, Raffaella De Maria, Paolo Accornero |
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| Rok vydání: | 2018 |
| Předmět: |
Gene isoform
comparative oncology 040301 veterinary sciences Mammary Neoplasms Animal Biology Cat Diseases Receptor tyrosine kinase Feline Mammary Carcinoma 0403 veterinary science 03 medical and health sciences Mammary Glands Animal Breast cancer Complementary DNA medicine Animals short form of RON 030304 developmental biology 0303 health sciences General Veterinary feline mammary tumors Reverse Transcriptase Polymerase Chain Reaction cats Intron Receptor Protein-Tyrosine Kinases Cancer cats feline mammary tumors short form of RON comparative oncology prognosis Sequence Analysis DNA 04 agricultural and veterinary sciences Prognosis medicine.disease Survival Analysis Molecular biology Cats biology.protein Immunohistochemistry Female |
| Zdroj: | Veterinary Pathology. 56:220-229 |
| ISSN: | 1544-2217 0300-9858 |
| Popis: | RON is a tyrosine kinase receptor activated by the macrophage-stimulating protein (MSP) ligand that is overexpressed in human breast cancer. In humans, RON protein can be present in different isoforms, and the most studied isoform is represented by the short form of RON ( sf-RON), which is generated by an alternative promoter located in intron 10 of the RON complementary DNA (cDNA). It plays an important role in breast cancer progression. Considering the many similarities between feline mammary carcinoma (FMC) and human breast cancer, the aim of this study was to investigate the expression of both RON and MSP in FMCs and to identify the presence of the sf-RON transcript. Tissue samples of spontaneous mammary tumors were collected from 60 queens (10 benign lesions, 50 carcinomas). All of the samples were tested for RON and MSP expression by immunohistochemistry; moreover, RNA was extracted from paraffin-embedded tissue samples, and the cDNA was tested by reverse transcription–polymerase chain reaction (RT-PCR) to identify the presence of sf-RON. Immunohistochemistry detected the expression of RON and MSP in 34 of 50 (68%) and 29 of 50 (58%) FMCs, respectively. RT-PCR revealed the presence of the short-form in 18 of 47 (38%) FMCs. This form originates, as in humans, from an alternative promoter (P2), and it codes for the proper feline short form ( sf-RON). sf-RON expression was associated with poorly differentiated tumors and with a shorter disease-free ( P < .05; hazard ratio [HR], 2.2) period and a shorter survival ( P < .05; HR, 2.2). These results support FMC as a suitable model in comparative oncology and identify sf-RON expression as potential predictor of outcomes for this disease. |
| Databáze: | OpenAIRE |
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