Commissural neurons transgress the cns/pns boundary in absence of ventricular zone-derived netrin 1
| Autor: | Juan Antonio Moreno-Bravo, Alain Chédotal, Heike Blockus, Pavol Zelina, Sergi Roig Puiggros, Patrick Mehlen, Chloé Dominici |
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| Přispěvatelé: | Agence Nationale de la Recherche (France) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Central Nervous System
Male 0301 basic medicine Cerebellum animal structures Central nervous system Hindbrain Biology Commissural neurons Mice 03 medical and health sciences Neural Stem Cells Cell Movement Pregnancy Pontine neurons Peripheral Nervous System Netrin medicine Animals Molecular Biology Migration Dcc Mice Knockout Neurons fungi Neural tube Netrin-1 Commissure DCC Receptor Spinal cord Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure nervous system Female Neuron Neuroscience Developmental Biology |
| Popis: | During the development of the central nervous system (CNS), only motor axons project into peripheral nerves. Little is known about the cellular and molecular mechanisms that control the development of a boundary at the CNS surface and prevent CNS neuron emigration from the neural tube. It has previously been shown that a subset of spinal cord commissural axons abnormally invades sensory nerves in Ntn1 hypomorphic embryos and Dcc knockouts. However, whether netrin 1 also plays a similar role in the brain is unknown. In the hindbrain, precerebellar neurons migrate tangentially under the pial surface, and their ventral migration is guided by netrin 1. Here, we show that pontine neurons and inferior olivary neurons, two types of precerebellar neurons, are not confined to the CNS in Ntn1 and Dcc mutant mice, but that they invade the trigeminal, auditory and vagus nerves. Using a Ntn1 conditional knockout, we show that netrin 1, which is released at the pial surface by ventricular zone progenitors is responsible for the CNS confinement of precerebellar neurons. We propose, that netrin 1 distribution sculpts the CNS boundary by keeping CNS neurons in netrin 1-rich domains. This work was supported by grants from the Agence Nationale de la Recherche (ANR-14-CE13-0004-01) (to A.C.). It was performed in the frame of the Labex Lifesenses (ANR-10-LABX-65) supported by French state funds managed by the Agence Nationale de la Recherche within the Investissements d'Avenir programme under ANR-11-IDEX-0004-02 (to A.C.). |
| Databáze: | OpenAIRE |
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