R-plasmid-encoded adhesive factor in Klebsiella pneumoniae strains responsible for human nosocomial infections
| Autor: | D Sirot, Arlette Darfeuille-Michaud, D Aubel, B Joly, C Jallat, J Sirot, C Rich |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.drug_class
Klebsiella pneumoniae R Factors Blotting Western Molecular Sequence Data Immunology Antibiotics Cross Reactions In Vitro Techniques medicine.disease_cause Microbiology Bacterial Adhesion chemistry.chemical_compound Plasmid Sequence Homology Nucleic Acid medicine Humans Escherichia coli Electrophoresis Agar Gel Cross Infection biology Nucleic Acid Hybridization Drug Resistance Microbial biology.organism_classification Enterobacteriaceae Intestines Microscopy Electron Infectious Diseases chemistry Colonic Neoplasms biology.protein Aerobactin Parasitology Antibody DNA Probes Polymorphism Restriction Fragment Length Bacteria Research Article Bacterial Outer Membrane Proteins |
| Zdroj: | Infection and Immunity. 60:44-55 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.60.1.44-55.1992 |
| Popis: | Klebsiella pneumoniae strains involved in hospital outbreaks of nosocomial infections, such as suppurative lesions, bacteremia, and septicemia, were resistant to multiple antibiotics including broad-spectrum cephalosporins. Epidemiologic investigations revealed that the reservoir for these K. pneumoniae strains was the gastrointestinal tracts of the patients. The study of the adherence ability of the strains reported here showed that these bacteria adhered to the microvilli of the Caco-2 cell line. This adhesion was mediated by a nonfimbrial protein with a molecular mass of 29,000 Da designated CF29K. Pretreatment of bacteria with antibodies raised against CF29K or Caco-2 cells with purified CF29K prevented the adhesion of K. pneumoniae strains to Caco-2 cells. CF29K immunologically cross-reacted with the CS31A surface protein of Escherichia coli strains involved in septicemia in calves. Genes encoding CF29K were located on a high-molecular-weight conjugative R plasmid, which transferred to E. coli K-12. Transconjugants expressed a large amount of CF29K protein and adhered to the brush border of Caco-2 cells. These findings show that K. pneumoniae strains were able to colonize the human intestinal tract through a plasmid-encoded 29,000-Da surface protein. Hybridization experiments indicated that the gene encoding resistance to broad-spectrum cephalosporins by the production of CAZ-1 enzyme and the gene encoding the adhesive property to intestinal cells were both located on a 20- to 22-kb EcoRI restriction DNA fragment. Genes encoding aerobactin and the ferric aerobactin receptor were also found on this R plasmid. |
| Databáze: | OpenAIRE |
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