Quantitative two-photon microscopy imaging analysis of human skin to evaluate enhanced transdermal delivery by hybrid-type multi-lamellar nanostructure
| Autor: | Kibaek Choe, Yeon Sook Kim, Kyeong Hu Kim, Pilhan Kim, Lee Seung Ki, Jinhyo Ahn, Joo Hyuck Lim |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Liposome
Nanostructure Materials science integumentary system Human skin 02 engineering and technology Permeation 021001 nanoscience & nanotechnology 01 natural sciences Atomic and Molecular Physics and Optics Article 010309 optics Two-photon excitation microscopy 0103 physical sciences Drug delivery Microscopy 0210 nano-technology Biotechnology Biomedical engineering Transdermal |
| Zdroj: | Biomed Opt Express |
| ISSN: | 2156-7085 |
| Popis: | Transdermal skin delivery is a method to transport various topical formulations to a deeper skin layer non-invasively. Permeability analysis of many delivering agents has been mostly conducted by a simple tape stripping method. However, it cannot reveal a detailed depth-dependent distribution profile of transdermally delivered agents in the skin. In this work, we achieved a cellular-level depth-defined visualization of fluorophore-labelled human epidermal growth factor (EGF) transdermally delivered to human skin by using encapsulation with common liposomes and newly fabricated multi-lamellar nanostructures using a custom-design two-photon microscopy system. It was able to generate 3D reconstructed images displaying the distribution of human EGF inside the human skin sample with high-resolution. Based on a depthwise fluorescence intensity profile showing the permeation of human EGF, a quantitative analysis was performed to assess the transdermal delivery efficacy achieved by each formulation, showing a significant improvement of the efficacy with the utilization of multi-lamellar nanostructure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|