Pathogen and Circadian Controlled 1 (PCC1) Protein Is Anchored to the Plasma Membrane and Interacts with Subunit 5 of COP9 Signalosome in Arabidopsis
| Autor: | Ricardo Mir, José León |
|---|---|
| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2014 |
| Předmět: |
Arabidopsis Thaliana
Protein subunit Arabidopsis Oligonucleotides lcsh:Medicine Plant Science Biology Real-Time Polymerase Chain Reaction Model Organisms Plant and Algal Models Gene Expression Regulation Plant RNA interference Two-Hybrid System Techniques Molecular Cell Biology COP9 signalosome lcsh:Science Transgenic Plants Gene Flowering Plants Plant Growth and Development Genetics Reporter gene Multidisciplinary Arabidopsis Proteins COP9 Signalosome Complex Cell Membrane fungi lcsh:R Gene Expression Regulation Developmental food and beverages Plants biology.organism_classification Phenotype Cell biology 81-amino acid protein Membrane protein Plant Physiology Plant Biotechnology Membranes and Sorting RNA Interference lcsh:Q Pathogen and Circadian Controlled 1 (PCC1) gene Research Article Biotechnology Developmental Biology |
| Zdroj: | PLoS ONE, Vol 9, Iss 1, p e87216 (2014) RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname PLoS ONE |
| DOI: | 10.1371/journal.pone.0087216 |
| Popis: | The Pathogen and Circadian Controlled 1 (PCC1) gene, previously identified and further characterized as involved in defense to pathogens and stress-induced flowering, codes for an 81-amino acid protein with a cysteine-rich C-terminal domain. This domain is essential for homodimerization and anchoring to the plasma membrane. Transgenic plants with the ß- glucuronidase (GUS) reporter gene under the control of 1.1 kb promoter sequence of PCC1 gene display a dual pattern of expression. At early post-germination, PCC1 is expressed only in the root vasculature and in the stomata guard cells of cotyledons. During the transition from vegetative to reproductive development, PCC1 is strongly expressed in the vascular tissue of petioles and basal part of the leaf, and it further spreads to the whole limb in fully expanded leaves. This developmental pattern of expression together with the late flowering phenotype of long-day grown RNA interference (iPCC1) plants with reduced PCC1 expression pointed to a regulatory role of PCC1 in the photoperiod-dependent flowering pathway. iPCC1 plants are defective in light perception and signaling but are not impaired in the function of the core CO-FT module of the photoperiod-dependent pathway. The regulatory effect exerted by PCC1 on the transition to flowering as well as on other reported phenotypes might be explained by a mechanism involving the interaction with the subunit 5 of the COP9 signalosome (CSN). This work was funded by grants BIO2008-00839, BIO2011-27526 and CSD2007-0057 from Ministerio de Ciencia e Innovacion of Spain to J.L. A fellowship/contract of the FPU program of the Ministerio de Educacion y Ciencia (Spain) funded R.M. work. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
| Databáze: | OpenAIRE |
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