Synthesis of δ-Aminolaevulinate Synthase in vitro Using Hepatic mRNA from Chick Embryos with Induced Porphyria
| Autor: | Brian K. May, J.D. Brooker, W.H. Elliott |
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| Rok vydání: | 2005 |
| Předmět: |
Polyadenylation
Chick Embryo Biology Biochemistry Hepatic porphyria Cell-free system Porphyrias Protein biosynthesis Animals RNA Messenger chemistry.chemical_classification Messenger RNA Cell-Free System ATP synthase Liver Diseases Nucleic Acid Hybridization RNA DNA Molecular biology Enzyme Liver chemistry Protein Biosynthesis biology.protein Chemical and Drug Induced Liver Injury Poly A 5-Aminolevulinate Synthetase |
| Zdroj: | European Journal of Biochemistry. 106:17-24 |
| ISSN: | 1432-1033 0014-2956 |
| Popis: | Polyadenylated mRNA was isolated from chick embryo liver following induction of hepatic porphyria. The RNA was translated in vitro using a wheat germ cell-free system and delta-aminolaevulinate synthase was identified in the translation products by indirect immunoprecipitation. The enzyme was not apparent in the translation products of polyadenylated RNA from non-induced livers. The molecular weight of delta-aminolaevulinate synthase synthesized in vitro was 70000 and the protein was estimated to represent up to 5% of total products synthesised in vitro. These data demonstrate for the first time that induction of chick embryo liver delta-aminolaevulinate synthase activity in hepatic porphyria correlates with a large increase in the translational capacity of isolated polyadenylated RNA for this enzyme and, together with preliminary cDNA . RNA hybridization studies, indicate that an increase in the level of delta-aminolaevulinic synthase mRNA is responsible. |
| Databáze: | OpenAIRE |
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