Glycogen Synthase Kinase-3 is involved in glycogen metabolism control and embryogenesis of Rhodnius prolixus
| Autor: | Marilvia Dansa, Hatisaburo Masuda, Glória R.C. Braz, Marcio Fontenele, Helena Araujo, Magda D. Lugon, Carlos Logullo, Rodrigo Nunes da Fonseca, Leonardo Araujo de Abreu, Mateus Berni, José Reinaldo Silva, Flavia Borges Mury |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Trypanosoma rangeli Indoles Rhodnius Embryonic Development 03 medical and health sciences chemistry.chemical_compound Glycogen Synthase Kinase 3 0302 clinical medicine Oogenesis GSK-3 Animals Gene Silencing Enzyme Inhibitors Rhodnius prolixus Gene knockdown biology Glycogen Gene Expression Profiling Wnt signaling pathway Insect physiology Benzazepines biology.organism_classification Cell biology 030104 developmental biology Infectious Diseases chemistry Animal Science and Zoology Parasitology 030217 neurology & neurosurgery |
| Zdroj: | Parasitology. 143(12) |
| ISSN: | 1469-8161 |
| Popis: | SUMMARYRhodnius prolixus is a blood-feeding insect that transmits Trypanosoma cruzi and Trypanosoma rangeli to vertebrate hosts. Rhodnius prolixus is also a classical model in insect physiology, and the recent availability of R. prolixus genome has opened new avenues on triatomine research. Glycogen synthase kinase 3 (GSK-3) is classically described as a key enzyme involved in glycogen metabolism, also acting as a downstream component of the Wnt pathway during embryogenesis. GSK-3 has been shown to be highly conserved among several organisms, mainly in the catalytic domain region. Meanwhile, the role of GSK-3 during R. prolixus embryogenesis or glycogen metabolism has not been investigated. Here we show that chemical inhibition of GSK-3 by alsterpaullone, an ATP-competitive inhibitor of GSK3, does not affect adult survival rate, though it alters oviposition and egg hatching. Specific GSK-3 gene silencing by dsRNA injection in adult females showed a similar phenotype. Furthermore, bright field and 4’−6-diamidino-2-phenylindole (DAPI) staining analysis revealed that ovaries and eggs from dsGSK-3 injected females exhibited specific morphological defects. We also demonstrate that glycogen content was inversely related to activity and transcription levels of GSK-3 during embryogenesis. Lastly, after GSK-3 knockdown, we observed changes in the expression of the Wingless (Wnt) downstream target β-catenin as well as in members of other pathways such as the receptor Notch. Taken together, our results show that GSK-3 regulation is essential for R. prolixus oogenesis and embryogenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|