Loss of ribosomal RNA modification causes developmental defects in zebrafish

Autor:	Naoko Hirano, Sayomi Higa-Nakamine, Anirban Chakraborty, Naoya Kenmochi, Yukari Nakajima, Mikako Nakamura, Tsutomu Suzuki, Tamayo Uechi, Takeo Suzuki
Jazyk:	angličtina
Rok vydání:	2011
Předmět:	Genetics urogenital system RNA Ribosomal RNA Biology biology.organism_classification Non-coding RNA Methylation Long non-coding RNA Morpholinos RRNA modification RNA Ribosomal RNA splicing Animals RNA Small Nucleolar Small nucleolar RNA RNA Processing Post-Transcriptional Zebrafish
Zdroj:	Nucleic Acids Research
ISSN:	1362-4962 0305-1048
Popis:	Non-coding RNAs (ncRNAs) play key roles in diverse cellular activities, and efficient ncRNA function requires extensive posttranscriptional nucleotide modifications. Small nucleolar RNAs (snoRNAs) are a group of ncRNAs that guide the modification of specific nucleotides in ribosomal RNAs (rRNAs) and small nuclear RNAs. To investigate the physiological relevance of rRNA modification in vertebrates, we suppressed the expression of three snoRNAs (U26, U44 and U78), either by disrupting the host gene splicing or by inhibiting the snoRNA precursor processing, and analyzed the consequences of snoRNA loss-of-function in zebrafish. Using a highly sensitive mass spectrometric analysis, we found that decreased snoRNA expression reduces the snoRNA-guided methylation of the target nucleotides. Impaired rRNA modification, even at a single site, led to severe morphological defects and embryonic lethality in zebrafish, which suggests that rRNA modifications play an essential role in vertebrate development. This study highlights the importance of posttranscriptional modifications and their role in ncRNA function in higher eukaryotes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b639431348bb77bce31736d6c6fee90f http://europepmc.org/articles/PMC3245925 Zobrazit plný text záznamu