Mice lacking integrin β3 expression exhibit altered response to chronic stress
| Autor: | Keith F. Polston, Seth Varney, Tammy Jessen, Ana Carneiro |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
5-HIAA
5-Hydroxyindoleacetic acid Physiology LCL lymphoblastoid cell lines Integrin Biochemistry lcsh:RC346-429 Open field FST forced swim test Endocrinology PCR polymerase chain reaction ECL electrochemiluminescence TBS tris-buffered saline Syntaxin Chronic stress Original Research Article ITGB3 ANOVA analysis of variance UCMS unpredictable chronic mild stress 5-HT 5-hydroxytryptamine biology Chemistry Depression lcsh:QP351-495 Glutamate receptor AMPA α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid ECM extracellular matrix ERK extracellular signal-related kinase NMDA N-Methyl-d-aspartic acid FAK focal adhesion kinase NMDA receptor Glutamate medicine.medical_specialty Serotonin PVDF polyvinylidene fluoride OFT open field test PBS phosphate-buffered saline AMPA receptor SEM standard error of the mean HPLC high pressure liquid chromatography lcsh:RC321-571 A.U. arbitrary units Cellular and Molecular Neuroscience Internal medicine PSD-95 post-synaptic density 95 protein medicine HVA homovanillic acid lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Molecular Biology lcsh:Neurology. Diseases of the nervous system Endocrine and Autonomic Systems HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid WT wild-type DOPAC 3 4-Dihydroxyphenylacetic acid EPM elevated plus maze PP2a protein phosphatase 2a lcsh:Neurophysiology and neuropsychology biology.protein Synaptophysin BSA bovine serum albumin Neuroscience Src c-Src tyrosine kinase |
| Zdroj: | Neurobiology of Stress Neurobiology of Stress, Vol 2, Iss C, Pp 51-58 (2015) |
| ISSN: | 2352-2895 |
| Popis: | Recent studies indicate multiple roles for integrin αvβ3 in adult neurons, including response to pharmacological agents such as cocaine and selective serotonin reuptake inhibitors. In this study, we examined the role of the integrin β3 gene (Itgb3) in the response to environmental stimuli by subjecting Itgb3+/+ and Itgb3−/− mice to unpredictable chronic mild stressors. We found that genetic abrogation of integrin β3 expression elicits an exaggerated vulnerability to chronic unpredictable stress in the open field test. In this test, chronic stress elicited significant decreases in stereotypic behavior and horizontal locomotor activity, including increases in anxiety behaviors. Mild chronic stress led to reductions in dopamine turnover in midbrains of Itgb3+/+, but not Itgb3−/− mice, suggesting a disruption of stress-dependent regulation of DA homeostasis. Chronic stress elicited altered synaptic expression of syntaxin and synaptophysin in midbrains of Itgb3−/− mice, when compared to Itgb3+/+. Semi-quantitative Western blot studies revealed that the synaptic expression, but not total tissue expression, of multiple signaling proteins is correlated with integrin αv levels in the midbrain. Moreover, loss of integrin β3 expression modifies this correlation network. Together, these findings demonstrate that Itgb3−/− mice display a pattern of changes indicating disrupted regulation of midbrain synaptic systems involved in conferring resilience to mild stressors. |
| Databáze: | OpenAIRE |
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