Wilms' tumour 1 (WT1) negatively regulates the expression of connexin 43 via a non-canonical Wnt signalling pathway in cultured bovine Sertoli cells
| Autor: | Mingjun Ma, Fushuo Huang, S.O. Adeniran, Xue Wang, Han Zhang, Ziming Wang, Peng Zheng, Xiaoyu Li, Guixue Zhang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
Cellular differentiation Connexin Endogeny Reproductive technology Biology 03 medical and health sciences 0302 clinical medicine Endocrinology Downregulation and upregulation Genetics medicine Animals WT1 Proteins Molecular Biology Wnt Signaling Pathway Cells Cultured 030304 developmental biology 0303 health sciences Gene knockdown 030219 obstetrics & reproductive medicine Sertoli Cells integumentary system Cell Differentiation Sertoli cell Hedgehog signaling pathway Cell biology medicine.anatomical_structure nervous system Reproductive Medicine Gene Expression Regulation Connexin 43 cardiovascular system Animal Science and Zoology Cattle sense organs tissues Developmental Biology Biotechnology |
| Zdroj: | Reproduction, fertility, and development. 32(5) |
| ISSN: | 1031-3613 |
| Popis: | The gap junction protein connexin (Cx) 43 between adjacent Sertoli cells (SCs) is the main testicular factor regulating the growth and development of SCs, and plays a vital role in controlling cell differentiation and maturation. However, the endogenous testicular factors that regulate Cx43 and the downstream signalling pathways that mediate Cx43-dependent SC differentiation are unclear. In this study, high-purity SCs were isolated from newborn calves’ testes by differential adherence. The SCs were then cultured invitro and treated with short interference RNA to knockdown endogenous Wilms’ tumour 1 (WT1). In WT1-knockdown SCs, Cx43 expression was upregulated. To elucidate the intracellular signalling mechanism of Cx43 in the differentiation and maturation of immature SCs, SCs were treated simultaneously with non-canonical Wnt signalling inhibitors CCG-1423 and GO-6983; in these SCs, Cx43 expression was upregulated. Together, these data indicate that WT1 negatively regulates the expression of Cx43 produced from SCs via a non-canonical Wnt signalling pathway in cultured bovine SCs. |
| Databáze: | OpenAIRE |
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