In Vitro and In Vivo Effect of 5-FC Combined Gene Therapy with TNF-α and CD Suicide Gene on Human Laryngeal Carcinoma Cell Line Hep-2
| Autor: | Dan Chen, Zhang-Feng Wang, Anxun Wang, Wei-Ying Liang, Liping Chai, Zhao-Qiang Zhang, Lei Chen |
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| Rok vydání: | 2013 |
| Předmět: |
Genetic enhancement
Cell Cancer Treatment Flucytosine Cytosine Deaminase Mice Basic Cancer Research Cloning Molecular Mice Inbred BALB C Multidisciplinary Reverse Transcriptase Polymerase Chain Reaction Genes Transgenic Suicide Gene Therapy Genomics Transfection Head and Neck Tumors medicine.anatomical_structure Oncology Carcinoma Squamous Cell Medicine Tumor necrosis factor alpha Research Article Science Genetic Vectors Mice Nude Biology Genomic Medicine In vivo Cell Line Tumor Genetics Cancer Genetics Escherichia coli medicine Animals Humans Laryngeal Neoplasms DNA Primers Clinical Genetics Analysis of Variance Tumor Necrosis Factor-alpha Cell growth Cancers and Neoplasms Human Genetics Genetic Therapy Suicide gene Molecular biology Otorhinolaryngology Head and Neck Cancers Cell culture |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 4, p e61136 (2013) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0061136 |
| Popis: | This study was aimed to investigate the effect of combined cancer gene therapy with exogenous tumor necrosis factor-alpha (TNF-α) and cytosine deaminase (CD) suicide gene on laryngeal carcinoma cell line Hep-2 in vitro and in vivo. Transfection of the recombinant eukaryotic vectors of pcDNA3.1 (+) containing TNF-α and/or CD into Hep-2 cells resulted in expression of TNF-α and/or CD gene in vitro. The significant increase in apoptotic Hep-2 cells and decrease of Hep-2 cell proliferation were observed using 5-FC treatment combined with TNF-a expression by CD/5-FC suicide system. Moreover, bystander effect was also observed in the TNF-α and CD gene co-expression group. Laryngeal squamous cell carcinoma (LSCC) mice model was established by using BALB/c mice which different transfected Hep-2 cells with pcDNA3.1 (+) containing TNF-α and/or CD were applied subcutaneously. So these mice are divided into four groups, namely, (1)Hep-2/TIC group; (2)Hep-2/CD group; (3)Hep-2/TNF-α group; (4)Hep-2/0 group. At day 29 after cell inoculation, volume of grafted tumor had significant difference between each two of them (P |
| Databáze: | OpenAIRE |
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