ANTIMICROBIAL ACTIVITY OF ANTIBIOTIC PASTES USED IN PULP THERAPY THROUGH DIRECT CONTACT WITH A MULTISPECIES BIOFILM: A PILOT STUDY
| Autor: | Maysa Lannes Duarte, Andréa Vaz Braga Pintor, Mariana Coutinho Sancas, Laura Guimarães Primo, Amanda Souza Nunes Monteiro, Anne Caroline Lunardi de Souza |
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| Jazyk: | portugalština |
| Rok vydání: | 2020 |
| Předmět: |
biology
Serial dilution testes de sensibilidade microbiana Chemistry Chloramphenicol microbial sensitivity tests Chlorhexidine Biofilm drug combination agentes antibacterianos antibacterial agents General Medicine biology.organism_classification Antimicrobial Enterococcus faecalis combinação de medicamentos Ciprofloxacin tratamento de canal radicular dente decíduo medicine root canal treatment Food science Candida albicans deciduous tooth medicine.drug |
| Zdroj: | Revista Científica do CRO-RJ (Rio de Janeiro Dental Journal); Vol. 5 No. 1: January-Abril 2020; 17-23 Revista Científica do CRO-RJ (Rio de Janeiro Dental Journal); v. 5 n. 1: January-Abril 2020; 17-23 Revista Científica do CRO-RJ Conselho Regional de Odontologia do Rio de Janeiro (CRO-RJ) instacron:CRO |
| ISSN: | 2595-4733 1518-5249 |
| DOI: | 10.29327/24816.5.1 |
| Popis: | Objective: To evaluate the antimicrobial activity of antibiotic pastes used in lesion sterilization and tissue repair (LSTR) technique, through a novel membrane direct contact methodology against a multispecies biofilm and to establish appropriate dilutions for this method. Methods: CTZ (chloramphenicol, tetracycline, zinc oxide) and two formulations of 3Mix pastes (ciprofloxacin, metronidazole, and minocycline), 3Mix1 and 3Mix3, were evaluated with negative (0.9% saline) and positive (chlorhexidine 0.2%) control groups. Candida albicans and Enterococcus faecalis (24-hour) biofilms (n=10) grown on cellulose membranes were directlyexposed to standardized amounts of fresh pastes and control solutions (n=2) for 24h. Membranes were immersed in 900 ?l of saline solution, and seven serial dilutions were made for each sample. Plating for each dilution (n=2) was performed on culture media for microbial colony-forming unit (CFU) counting of totalmicroorganisms, Candida spp. and Enterococcus spp. Aiming the comparison between groups, CFU quantification data were transformed into log10 CFU / mL and the Mann-Whitney test was applied (p |
| Databáze: | OpenAIRE |
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