Mirrored pyramidal wells for simultaneous multiple vantage point microscopy
| Autor: | Charles T. Wright, John P. Wikswo, Kevin T. Seale, I.A. Ges, Dmitry A. Markov, Chris Janetopoulos, Ron S. Reiserer |
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| Rok vydání: | 2008 |
| Předmět: |
Organelles
Microscopy Fluorescence-lifetime imaging microscopy Histology Photon Materials science business.industry Vantage point 3D reconstruction Tracking (particle physics) Article Specimen Handling Pathology and Forensic Medicine law.invention Imaging Three-Dimensional Optics Microscopy Fluorescence Confocal microscopy law Animals Dictyostelium business |
| Zdroj: | Journal of Microscopy. 232:1-6 |
| ISSN: | 1365-2818 0022-2720 |
| DOI: | 10.1111/j.1365-2818.2008.02110.x |
| Popis: | We report a novel method for obtaining simultaneous images from multiple vantage points of a microscopic specimen using size-matched microscopic mirrors created from anisotropically etched silicon. The resulting pyramidal wells enable bright-field and fluorescent side-view images, and when combined with z-sectioning, provide additional information for 3D reconstructions of the specimen. We have demonstrated the 3D localization and tracking over time of the centrosome of a live Dictyostelium discoideum. The simultaneous acquisition of images from multiple perspectives also provides a five-fold increase in the theoretical collection efficiency of emitted photons, a property which may be useful for low-light imaging modalities such as bioluminescence, or low abundance surface-marker labelling. |
| Databáze: | OpenAIRE |
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